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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Klebsiella pneumoniae Glucans biosynthesis glucosyltransferase H (mdoH), partial CSB-YP466663KBH
CSB-EP466663KBH
CSB-BP466663KBH
CSB-MP466663KBH
CSB-EP466663KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Putative aminoacrylate peracid reductase RutC (rutC) CSB-YP466664KBH
CSB-EP466664KBH
CSB-BP466664KBH
CSB-MP466664KBH
CSB-EP466664KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP466665KBH
CSB-EP466665KBH
CSB-BP466665KBH
CSB-MP466665KBH
CSB-EP466665KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Ribosomal RNA large subunit methyltransferase I (rlmI) CSB-YP466666KBH
CSB-EP466666KBH
CSB-BP466666KBH
CSB-MP466666KBH
CSB-EP466666KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Chromosome partition protein mukF (mukF) CSB-YP466667KBH
CSB-EP466667KBH
CSB-BP466667KBH
CSB-MP466667KBH
CSB-EP466667KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Outer-membrane lipoprotein carrier protein (lolA) CSB-BP466668KBH
CSB-MP466668KBH
CSB-EP466668KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Isocitrate dehydrogenase kinase/phosphatase (aceK), partial CSB-YP466669KBH
CSB-EP466669KBH
CSB-BP466669KBH
CSB-MP466669KBH
CSB-EP466669KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 50S ribosomal protein L7/L12 (rplL) CSB-YP466670KBH
CSB-EP466670KBH
CSB-BP466670KBH
CSB-MP466670KBH
CSB-EP466670KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Protein CyaY (cyaY) CSB-YP466671KBH
CSB-EP466671KBH
CSB-BP466671KBH
CSB-MP466671KBH
CSB-EP466671KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP466672KBH
CSB-EP466672KBH
CSB-BP466672KBH
CSB-MP466672KBH
CSB-EP466672KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Soluble pyridine nucleotide transhydrogenase (sthA) CSB-YP466673KBH
CSB-EP466673KBH
CSB-BP466673KBH
CSB-MP466673KBH
CSB-EP466673KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP466674KBH
CSB-EP466674KBH
CSB-BP466674KBH
CSB-MP466674KBH
CSB-EP466674KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP466675KBH
CSB-EP466675KBH
CSB-BP466675KBH
CSB-MP466675KBH
CSB-EP466675KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Rhamnulokinase (rhaB) CSB-YP466676KBH
CSB-EP466676KBH
CSB-BP466676KBH
CSB-MP466676KBH
CSB-EP466676KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 8-amino-7-oxononanoate synthase (bioF) CSB-YP466677KBH
CSB-EP466677KBH
CSB-BP466677KBH
CSB-MP466677KBH
CSB-EP466677KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Imidazolonepropionase (hutI) CSB-YP466678KBH
CSB-EP466678KBH
CSB-BP466678KBH
CSB-MP466678KBH
CSB-EP466678KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Potassium-transporting ATPase A chain (kdpA), partial CSB-YP466679KBH
CSB-EP466679KBH
CSB-BP466679KBH
CSB-MP466679KBH
CSB-EP466679KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Protein FdhD homolog (fdhD) CSB-YP466680KBH
CSB-EP466680KBH
CSB-BP466680KBH
CSB-MP466680KBH
CSB-EP466680KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Bifunctional protein GlmU (glmU) CSB-YP466681KBH
CSB-EP466681KBH
CSB-BP466681KBH
CSB-MP466681KBH
CSB-EP466681KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Probable rRNA maturation factor (KPK_3889) CSB-YP466682KBH
CSB-EP466682KBH
CSB-BP466682KBH
CSB-MP466682KBH
CSB-EP466682KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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