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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus Putative phosphotransferase BCAH820_4318 (BCAH820_4318) CSB-YP467803BQM
CSB-EP467803BQM
CSB-BP467803BQM
CSB-MP467803BQM
CSB-EP467803BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Processive diacylglycerol glucosyltransferase (ugtP) CSB-YP467804BQM
CSB-EP467804BQM
CSB-BP467804BQM
CSB-MP467804BQM
CSB-EP467804BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0295 protein BCAH820_0521 (BCAH820_0521), partial CSB-YP467805BQM1
CSB-EP467805BQM1
CSB-BP467805BQM1
CSB-MP467805BQM1
CSB-EP467805BQM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase, partial CSB-YP467806BQM
CSB-EP467806BQM
CSB-BP467806BQM
CSB-MP467806BQM
CSB-EP467806BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP467807BQM
CSB-EP467807BQM
CSB-BP467807BQM
CSB-MP467807BQM
CSB-EP467807BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Trigger factor (tig) CSB-YP467808BQM
CSB-EP467808BQM
CSB-BP467808BQM
CSB-MP467808BQM
CSB-EP467808BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UvrABC system protein C (uvrC), partial CSB-YP467809BQM
CSB-EP467809BQM
CSB-BP467809BQM
CSB-MP467809BQM
CSB-EP467809BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L20 (rplT) CSB-YP467810BQM
CSB-EP467810BQM
CSB-BP467810BQM
CSB-MP467810BQM
CSB-EP467810BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Potassium-transporting ATPase C chain (kdpC), partial CSB-YP467811BQM1
CSB-EP467811BQM1
CSB-BP467811BQM1
CSB-MP467811BQM1
CSB-EP467811BQM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP467812BQM
CSB-EP467812BQM
CSB-BP467812BQM
CSB-MP467812BQM
CSB-EP467812BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S4 (rpsD) CSB-YP467813BQM
CSB-EP467813BQM
CSB-BP467813BQM
CSB-MP467813BQM
CSB-EP467813BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0342 protein BCAH820_0955 (BCAH820_0955) CSB-YP467814BQM
CSB-EP467814BQM
CSB-BP467814BQM
CSB-MP467814BQM
CSB-EP467814BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable molybdopterin-guanine dinucleotide biosynthesis protein A (mobA) CSB-YP467815BQM
CSB-EP467815BQM
CSB-BP467815BQM
CSB-MP467815BQM
CSB-EP467815BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0751 protein BCAH820_B0138 (BCAH820_B0138) CSB-YP467816BQM
CSB-EP467816BQM
CSB-BP467816BQM
CSB-MP467816BQM
CSB-EP467816BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Histidinol-phosphate aminotransferase (hisC) CSB-YP467817EPW
CSB-EP467817EPW
CSB-BP467817EPW
CSB-MP467817EPW
CSB-EP467817EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Light-independent protochlorophyllide reductase subunit B (chlB), partial CSB-YP467818EPW
CSB-EP467818EPW
CSB-BP467818EPW
CSB-MP467818EPW
CSB-EP467818EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Bifunctional protein GlmU (glmU) CSB-YP467819EPW
CSB-EP467819EPW
CSB-BP467819EPW
CSB-MP467819EPW
CSB-EP467819EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP467820EPW
CSB-EP467820EPW
CSB-BP467820EPW
CSB-MP467820EPW
CSB-EP467820EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Chorismate synthase (aroC) CSB-YP467821EPW
CSB-EP467821EPW
CSB-BP467821EPW
CSB-MP467821EPW
CSB-EP467821EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Enolase (eno) CSB-YP467822EPW
CSB-EP467822EPW
CSB-BP467822EPW
CSB-MP467822EPW
CSB-EP467822EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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