Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli UPF0213 protein yhbQ (yhbQ) CSB-YP467063ENW
CSB-EP467063ENW
CSB-BP467063ENW
CSB-MP467063ENW
CSB-EP467063ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP467064ENW
CSB-EP467064ENW
CSB-BP467064ENW
CSB-MP467064ENW
CSB-EP467064ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP467065ENW1
CSB-EP467065ENW1
CSB-BP467065ENW1
CSB-MP467065ENW1
CSB-EP467065ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L13 (rplM) CSB-YP467066ENW
CSB-EP467066ENW
CSB-BP467066ENW
CSB-MP467066ENW
CSB-EP467066ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosomal protein L11 methyltransferase (prmA) CSB-YP467067ENW
CSB-EP467067ENW
CSB-BP467067ENW
CSB-MP467067ENW
CSB-EP467067ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L17 (rplQ) CSB-YP467068ENW
CSB-EP467068ENW
CSB-BP467068ENW
CSB-MP467068ENW
CSB-EP467068ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L18 (rplR) CSB-YP467069ENW
CSB-EP467069ENW
CSB-BP467069ENW
CSB-MP467069ENW
CSB-EP467069ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L14 (rplN) CSB-YP467070ENW
CSB-EP467070ENW
CSB-BP467070ENW
CSB-MP467070ENW
CSB-EP467070ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L4 (rplD) CSB-YP467071ENW
CSB-EP467071ENW
CSB-BP467071ENW
CSB-MP467071ENW
CSB-EP467071ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein TusB (tusB) CSB-YP467072ENW
CSB-EP467072ENW
CSB-BP467072ENW
CSB-MP467072ENW
CSB-EP467072ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein YjeA (yjeA) CSB-YP467073ENW
CSB-EP467073ENW
CSB-BP467073ENW
CSB-MP467073ENW
CSB-EP467073ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Adenylosuccinate synthetase (purA) CSB-YP467074ENW
CSB-EP467074ENW
CSB-BP467074ENW
CSB-MP467074ENW
CSB-EP467074ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 30S ribosomal protein S6 (rpsF) CSB-YP467075ENW
CSB-EP467075ENW
CSB-BP467075ENW
CSB-MP467075ENW
CSB-EP467075ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli MgtA leader peptide (mgtL) CSB-YP467076ENW
CSB-EP467076ENW
CSB-BP467076ENW
CSB-MP467076ENW
CSB-EP467076ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP467077ENW
CSB-EP467077ENW
CSB-BP467077ENW
CSB-MP467077ENW
CSB-EP467077ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0270 protein YheU (yheU) CSB-YP467078ENW
CSB-EP467078ENW
CSB-BP467078ENW
CSB-MP467078ENW
CSB-EP467078ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Pimelyl-[acyl-carrier protein] methyl ester esterase (bioH) CSB-YP467079ENW
CSB-EP467079ENW
CSB-BP467079ENW
CSB-MP467079ENW
CSB-EP467079ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP467080ENW
CSB-EP467080ENW
CSB-BP467080ENW
CSB-MP467080ENW
CSB-EP467080ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli C4-dicarboxylate transport protein (dctA), partial CSB-YP467081ENW
CSB-EP467081ENW
CSB-BP467081ENW
CSB-MP467081ENW
CSB-EP467081ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP467082ENW
CSB-EP467082ENW
CSB-BP467082ENW
CSB-MP467082ENW
CSB-EP467082ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>