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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vibrio fischeri Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP468603VFC
CSB-EP468603VFC
CSB-BP468603VFC
CSB-MP468603VFC
CSB-EP468603VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Chaperone protein DnaJ (dnaJ) CSB-YP468604SWN
CSB-EP468604SWN
CSB-BP468604SWN
CSB-MP468604SWN
CSB-EP468604SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Probable sugar efflux transporter (sotB), partial CSB-YP468605SWN
CSB-EP468605SWN
CSB-BP468605SWN
CSB-MP468605SWN
CSB-EP468605SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Spermidine export protein MdtI (mdtI), partial CSB-YP468606SWN1
CSB-EP468606SWN1
CSB-BP468606SWN1
CSB-MP468606SWN1
CSB-EP468606SWN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP468607SWN
CSB-EP468607SWN
CSB-BP468607SWN
CSB-MP468607SWN
CSB-EP468607SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin DnaA initiator-associating protein diaA (diaA) CSB-YP468608SWN
CSB-EP468608SWN
CSB-BP468608SWN
CSB-MP468608SWN
CSB-EP468608SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP468609SWN
CSB-EP468609SWN
CSB-BP468609SWN
CSB-MP468609SWN
CSB-EP468609SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin DNA gyrase inhibitor YacG (yacG) CSB-YP468610SWN
CSB-EP468610SWN
CSB-BP468610SWN
CSB-MP468610SWN
CSB-EP468610SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Electron transport complex protein RnfE (rnfE), partial CSB-YP468611SWN1
CSB-EP468611SWN1
CSB-BP468611SWN1
CSB-MP468611SWN1
CSB-EP468611SWN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Secretion system apparatus protein SsaV (ssaV), partial CSB-YP468612SWN1
CSB-EP468612SWN1
CSB-BP468612SWN1
CSB-MP468612SWN1
CSB-EP468612SWN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0042 nucleotide-binding protein yhbJ (yhbJ) CSB-YP468613SWN
CSB-EP468613SWN
CSB-BP468613SWN
CSB-MP468613SWN
CSB-EP468613SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Arginine repressor (argR) CSB-YP468614SWN
CSB-EP468614SWN
CSB-BP468614SWN
CSB-MP468614SWN
CSB-EP468614SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin DNA-binding protein fis (fis) CSB-YP468615SWN
CSB-EP468615SWN
CSB-BP468615SWN
CSB-MP468615SWN
CSB-EP468615SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP468616SWN
CSB-EP468616SWN
CSB-BP468616SWN
CSB-MP468616SWN
CSB-EP468616SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Ribonuclease H (rnhA) CSB-YP468617SWN
CSB-EP468617SWN
CSB-BP468617SWN
CSB-MP468617SWN
CSB-EP468617SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Integration host factor subunit alpha (ihfA) CSB-YP468618SWN
CSB-EP468618SWN
CSB-BP468618SWN
CSB-MP468618SWN
CSB-EP468618SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0249 protein ChbG (chbG) CSB-YP468619SWN
CSB-EP468619SWN
CSB-BP468619SWN
CSB-MP468619SWN
CSB-EP468619SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0229 protein yeaH (yeaH) CSB-YP468620SWN
CSB-EP468620SWN
CSB-BP468620SWN
CSB-MP468620SWN
CSB-EP468620SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Large-conductance mechanosensitive channel (mscL), partial CSB-YP468621SWN1
CSB-EP468621SWN1
CSB-BP468621SWN1
CSB-MP468621SWN1
CSB-EP468621SWN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 50S ribosomal protein L14 (rplN) CSB-YP468622SWN
CSB-EP468622SWN
CSB-BP468622SWN
CSB-MP468622SWN
CSB-EP468622SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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