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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oligotropha carboxidovorans Protein grpE (grpE) CSB-YP467223OED
CSB-EP467223OED
CSB-BP467223OED
CSB-MP467223OED
CSB-EP467223OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP467224OED
CSB-EP467224OED
CSB-BP467224OED
CSB-MP467224OED
CSB-EP467224OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Tryptophan synthase beta chain (trpB) CSB-YP467225OED
CSB-EP467225OED
CSB-BP467225OED
CSB-MP467225OED
CSB-EP467225OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans ATP synthase epsilon chain (atpC) CSB-YP467226OED
CSB-EP467226OED
CSB-BP467226OED
CSB-MP467226OED
CSB-EP467226OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 50S ribosomal protein L27 (rpmA) CSB-YP467227OED
CSB-EP467227OED
CSB-BP467227OED
CSB-MP467227OED
CSB-EP467227OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Formyl-coenzyme A transferase (frc) CSB-YP467228OED
CSB-EP467228OED
CSB-BP467228OED
CSB-MP467228OED
CSB-EP467228OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP467229OED
CSB-EP467229OED
CSB-BP467229OED
CSB-MP467229OED
CSB-EP467229OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 30S ribosomal protein S19 (rpsS) CSB-YP467230OED
CSB-EP467230OED
CSB-BP467230OED
CSB-MP467230OED
CSB-EP467230OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 50S ribosomal protein L24 (rplX) CSB-YP467231OED
CSB-EP467231OED
CSB-BP467231OED
CSB-MP467231OED
CSB-EP467231OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 50S ribosomal protein L15 (rplO) CSB-YP467232OED
CSB-EP467232OED
CSB-BP467232OED
CSB-MP467232OED
CSB-EP467232OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP467233OED
CSB-EP467233OED
CSB-BP467233OED
CSB-MP467233OED
CSB-EP467233OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP467234OED
CSB-EP467234OED
CSB-BP467234OED
CSB-MP467234OED
CSB-EP467234OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Indole-3-glycerol phosphate synthase (trpC) CSB-YP467235OED
CSB-EP467235OED
CSB-BP467235OED
CSB-MP467235OED
CSB-EP467235OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP467236OED
CSB-EP467236OED
CSB-BP467236OED
CSB-MP467236OED
CSB-EP467236OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Aspartate--tRNA ligase (aspS), partial CSB-YP467237OED
CSB-EP467237OED
CSB-BP467237OED
CSB-MP467237OED
CSB-EP467237OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Phosphate acyltransferase (plsX) CSB-YP467238OED
CSB-EP467238OED
CSB-BP467238OED
CSB-MP467238OED
CSB-EP467238OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP467239OED
CSB-EP467239OED
CSB-BP467239OED
CSB-MP467239OED
CSB-EP467239OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP467240OED
CSB-EP467240OED
CSB-BP467240OED
CSB-MP467240OED
CSB-EP467240OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP467241OED
CSB-EP467241OED
CSB-BP467241OED
CSB-MP467241OED
CSB-EP467241OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 50S ribosomal protein L25 (rplY) CSB-YP467242OED
CSB-EP467242OED
CSB-BP467242OED
CSB-MP467242OED
CSB-EP467242OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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