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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodospirillum centenum N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP467163RLY
CSB-EP467163RLY
CSB-BP467163RLY
CSB-MP467163RLY
CSB-EP467163RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP467164RLY
CSB-EP467164RLY
CSB-BP467164RLY
CSB-MP467164RLY
CSB-EP467164RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Sugar fermentation stimulation protein homolog (sfsA) CSB-YP467165RLY
CSB-EP467165RLY
CSB-BP467165RLY
CSB-MP467165RLY
CSB-EP467165RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Exodeoxyribonuclease 7 large subunit (xseA), partial CSB-YP467166RLY
CSB-EP467166RLY
CSB-BP467166RLY
CSB-MP467166RLY
CSB-EP467166RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP467167DXM
CSB-EP467167DXM
CSB-BP467167DXM
CSB-MP467167DXM
CSB-EP467167DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Protein-export protein secB (secB) CSB-YP467168DXM
CSB-EP467168DXM
CSB-BP467168DXM
CSB-MP467168DXM
CSB-EP467168DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP467169DXM
CSB-EP467169DXM
CSB-BP467169DXM
CSB-MP467169DXM
CSB-EP467169DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Integration host factor subunit alpha (ihfA) CSB-YP467170DXM
CSB-EP467170DXM
CSB-BP467170DXM
CSB-MP467170DXM
CSB-EP467170DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP467171DXM
CSB-EP467171DXM
CSB-BP467171DXM
CSB-MP467171DXM
CSB-EP467171DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Probable Fe (2+)-trafficking protein CSB-YP467172DXM
CSB-EP467172DXM
CSB-BP467172DXM
CSB-MP467172DXM
CSB-EP467172DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Trigger factor (tig) CSB-YP467173DXM
CSB-EP467173DXM
CSB-BP467173DXM
CSB-MP467173DXM
CSB-EP467173DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Lipid-A-disaccharide synthase (lpxB) CSB-YP467174DXM
CSB-EP467174DXM
CSB-BP467174DXM
CSB-MP467174DXM
CSB-EP467174DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii DNA ligase (ligA), partial CSB-YP467175DXM
CSB-EP467175DXM
CSB-BP467175DXM
CSB-MP467175DXM
CSB-EP467175DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP467176DXM
CSB-EP467176DXM
CSB-BP467176DXM
CSB-MP467176DXM
CSB-EP467176DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP467177DXM
CSB-EP467177DXM
CSB-BP467177DXM
CSB-MP467177DXM
CSB-EP467177DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Phosphomethylpyrimidine synthase (thiC), partial CSB-YP467178DXM
CSB-EP467178DXM
CSB-BP467178DXM
CSB-MP467178DXM
CSB-EP467178DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L28 (rpmB) CSB-YP467179DXM
CSB-EP467179DXM
CSB-BP467179DXM
CSB-MP467179DXM
CSB-EP467179DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L30 (rpmD) CSB-YP467180DXM
CSB-EP467180DXM
CSB-BP467180DXM
CSB-MP467180DXM
CSB-EP467180DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 30S ribosomal protein S3 (rpsC) CSB-YP467181DXM
CSB-EP467181DXM
CSB-BP467181DXM
CSB-MP467181DXM
CSB-EP467181DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 30S ribosomal protein S7 (rpsG) CSB-YP467182DXM
CSB-EP467182DXM
CSB-BP467182DXM
CSB-MP467182DXM
CSB-EP467182DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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