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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Methylthioribulose-1-phosphate dehydratase (MDE1) CSB-YP468883SVH
CSB-EP468883SVH
CSB-BP468883SVH
CSB-MP468883SVH
CSB-EP468883SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial biogenesis regulation protein 1 (MBR1) CSB-YP468884SVH
CSB-EP468884SVH
CSB-BP468884SVH
CSB-MP468884SVH
CSB-EP468884SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Guanine nucleotide exchange factor SDC25 (SDC25), partial CSB-YP468885SVH
CSB-EP468885SVH
CSB-BP468885SVH
CSB-MP468885SVH
CSB-EP468885SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Arrestin-related trafficking adapter 10 (ART10), partial CSB-YP468886SVH
CSB-EP468886SVH
CSB-BP468886SVH
CSB-MP468886SVH
CSB-EP468886SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Eisosome protein 1 (EIS1), partial CSB-YP468887SVH
CSB-EP468887SVH
CSB-BP468887SVH
CSB-MP468887SVH
CSB-EP468887SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein YIM1 (YIM1) CSB-YP468888SVH
CSB-EP468888SVH
CSB-BP468888SVH
CSB-MP468888SVH
CSB-EP468888SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cysteine proteinase 1, mitochondrial (LAP3) CSB-YP468889SVH
CSB-EP468889SVH
CSB-BP468889SVH
CSB-MP468889SVH
CSB-EP468889SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Altered inheritance of mitochondria protein 37, mitochondrial (AIM37), partial CSB-YP468890SVH1
CSB-EP468890SVH1
CSB-BP468890SVH1
CSB-MP468890SVH1
CSB-EP468890SVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Lipoyl synthase, mitochondrial (LIP5) CSB-YP468891SVH
CSB-EP468891SVH
CSB-BP468891SVH
CSB-MP468891SVH
CSB-EP468891SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit (SPT14), partial CSB-YP468892SVH1
CSB-EP468892SVH1
CSB-BP468892SVH1
CSB-MP468892SVH1
CSB-EP468892SVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana K (+) efflux antiporter 6, partial CSB-YP468893DOA
CSB-EP468893DOA
CSB-BP468893DOA
CSB-MP468893DOA
CSB-EP468893DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Draxin-B CSB-YP468894SWI
CSB-EP468894SWI
CSB-BP468894SWI
CSB-MP468894SWI
CSB-EP468894SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Calumenin-B (calub) CSB-YP468895SWI
CSB-EP468895SWI
CSB-BP468895SWI
CSB-MP468895SWI
CSB-EP468895SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Peptidyl-tRNA hydrolase ICT1, mitochondrial (ict1) CSB-YP468896SWI
CSB-EP468896SWI
CSB-BP468896SWI
CSB-MP468896SWI
CSB-EP468896SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Ribosome maturation factor RimP (rimP) CSB-YP468897FNH
CSB-EP468897FNH
CSB-BP468897FNH
CSB-MP468897FNH
CSB-EP468897FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP468898FNH
CSB-EP468898FNH
CSB-BP468898FNH
CSB-MP468898FNH
CSB-EP468898FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 GTP-sensing transcriptional pleiotropic repressor CodY (codY) CSB-YP468899FNH
CSB-EP468899FNH
CSB-BP468899FNH
CSB-MP468899FNH
CSB-EP468899FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP468900FNH
CSB-EP468900FNH
CSB-BP468900FNH
CSB-MP468900FNH
CSB-EP468900FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 30S ribosomal protein S9 (rpsI) CSB-YP468901FNH
CSB-EP468901FNH
CSB-BP468901FNH
CSB-MP468901FNH
CSB-EP468901FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Chromosomal replication initiator protein DnaA (dnaA) CSB-YP468902FNH
CSB-EP468902FNH
CSB-BP468902FNH
CSB-MP468902FNH
CSB-EP468902FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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