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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cyanothece sp. Dihydrodipicolinate reductase (dapB) CSB-YP467863DZZ
CSB-EP467863DZZ
CSB-BP467863DZZ
CSB-MP467863DZZ
CSB-EP467863DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD1) CSB-YP467864AZX
CSB-EP467864AZX
CSB-BP467864AZX
CSB-MP467864AZX
CSB-EP467864AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Succinyl-diaminopimelate desuccinylase 1 (dapE1) CSB-YP467865AZX
CSB-EP467865AZX
CSB-BP467865AZX
CSB-MP467865AZX
CSB-EP467865AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP467866AZX
CSB-EP467866AZX
CSB-BP467866AZX
CSB-MP467866AZX
CSB-EP467866AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii tRNA (mo5U34)-methyltransferase CSB-YP467867AZX
CSB-EP467867AZX
CSB-BP467867AZX
CSB-MP467867AZX
CSB-EP467867AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 60 kDa chaperonin (groL), partial CSB-YP467868AZX
CSB-EP467868AZX
CSB-BP467868AZX
CSB-MP467868AZX
CSB-EP467868AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Tryptophan synthase beta chain (trpB) CSB-YP467869AZX
CSB-EP467869AZX
CSB-BP467869AZX
CSB-MP467869AZX
CSB-EP467869AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Shikimate dehydrogenase (aroE) CSB-YP467870AZX
CSB-EP467870AZX
CSB-BP467870AZX
CSB-MP467870AZX
CSB-EP467870AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii UPF0758 protein MADE_1000235 (MADE_1000235) CSB-YP467871AZX
CSB-EP467871AZX
CSB-BP467871AZX
CSB-MP467871AZX
CSB-EP467871AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP467872AZX
CSB-EP467872AZX
CSB-BP467872AZX
CSB-MP467872AZX
CSB-EP467872AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP467873AZX
CSB-EP467873AZX
CSB-BP467873AZX
CSB-MP467873AZX
CSB-EP467873AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Threonine--tRNA ligase (thrS) CSB-YP467874EYX
CSB-EP467874EYX
CSB-BP467874EYX
CSB-MP467874EYX
CSB-EP467874EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP467875EYX
CSB-EP467875EYX
CSB-BP467875EYX
CSB-MP467875EYX
CSB-EP467875EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii 50S ribosomal protein L32 (rpmF) CSB-YP467876EYX
CSB-EP467876EYX
CSB-BP467876EYX
CSB-MP467876EYX
CSB-EP467876EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Glutamate--tRNA ligase (gltX) CSB-YP467877EYX
CSB-EP467877EYX
CSB-BP467877EYX
CSB-MP467877EYX
CSB-EP467877EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii GTPase Der (der) CSB-YP467878EYX
CSB-EP467878EYX
CSB-BP467878EYX
CSB-MP467878EYX
CSB-EP467878EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii 50S ribosomal protein L10 (rplJ) CSB-YP467879EYX
CSB-EP467879EYX
CSB-BP467879EYX
CSB-MP467879EYX
CSB-EP467879EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP467880EYX
CSB-EP467880EYX
CSB-BP467880EYX
CSB-MP467880EYX
CSB-EP467880EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP467881EYX
CSB-EP467881EYX
CSB-BP467881EYX
CSB-MP467881EYX
CSB-EP467881EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Glycogen synthase (glgA) CSB-YP467882EYX
CSB-EP467882EYX
CSB-BP467882EYX
CSB-MP467882EYX
CSB-EP467882EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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