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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ureaplasma urealyticum serovar 10 50S ribosomal protein L19 (rplS) CSB-YP469223UBL
CSB-EP469223UBL
CSB-BP469223UBL
CSB-MP469223UBL
CSB-EP469223UBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ureaplasma urealyticum serovar 10 Deoxyribose-phosphate aldolase (deoC) CSB-YP469224UBL
CSB-EP469224UBL
CSB-BP469224UBL
CSB-MP469224UBL
CSB-EP469224UBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ureaplasma urealyticum serovar 10 Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP469225UBL
CSB-EP469225UBL
CSB-BP469225UBL
CSB-MP469225UBL
CSB-EP469225UBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii UPF0173 metal-dependent hydrolase Rleg2_1519 (Rleg2_1519) CSB-YP469226RKQ
CSB-EP469226RKQ
CSB-BP469226RKQ
CSB-MP469226RKQ
CSB-EP469226RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Urease accessory protein UreE (ureE) CSB-YP469227RKQ
CSB-EP469227RKQ
CSB-BP469227RKQ
CSB-MP469227RKQ
CSB-EP469227RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Apolipoprotein N-acyltransferase (lnt), partial CSB-YP469228RKQ1
CSB-EP469228RKQ1
CSB-BP469228RKQ1
CSB-MP469228RKQ1
CSB-EP469228RKQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Acetylglutamate kinase (argB) CSB-YP469229RKQ
CSB-EP469229RKQ
CSB-BP469229RKQ
CSB-MP469229RKQ
CSB-EP469229RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP469230RKQ
CSB-EP469230RKQ
CSB-BP469230RKQ
CSB-MP469230RKQ
CSB-EP469230RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Probable D-serine dehydratase (dsdA) CSB-YP469231RKQ
CSB-EP469231RKQ
CSB-BP469231RKQ
CSB-MP469231RKQ
CSB-EP469231RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii 50S ribosomal protein L31 (rpmE) CSB-YP469232RKQ
CSB-EP469232RKQ
CSB-BP469232RKQ
CSB-MP469232RKQ
CSB-EP469232RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP469233RKQ
CSB-EP469233RKQ
CSB-BP469233RKQ
CSB-MP469233RKQ
CSB-EP469233RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Threonine--tRNA ligase (thrS) CSB-YP469234RKQ
CSB-EP469234RKQ
CSB-BP469234RKQ
CSB-MP469234RKQ
CSB-EP469234RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Lysine--tRNA ligase (lysS) CSB-YP469235RKQ
CSB-EP469235RKQ
CSB-BP469235RKQ
CSB-MP469235RKQ
CSB-EP469235RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Ribonuclease HII (rnhB) CSB-YP469236RKQ
CSB-EP469236RKQ
CSB-BP469236RKQ
CSB-MP469236RKQ
CSB-EP469236RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Probable molybdopterin-guanine dinucleotide biosynthesis protein A (mobA) CSB-YP469237RKQ
CSB-EP469237RKQ
CSB-BP469237RKQ
CSB-MP469237RKQ
CSB-EP469237RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP469238RKQ
CSB-EP469238RKQ
CSB-BP469238RKQ
CSB-MP469238RKQ
CSB-EP469238RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Cytochrome c oxidase assembly protein CtaG (ctaG), partial CSB-YP469239RKQ
CSB-EP469239RKQ
CSB-BP469239RKQ
CSB-MP469239RKQ
CSB-EP469239RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii UPF0235 protein Rleg2_3707 (Rleg2_3707) CSB-YP469240RKQ
CSB-EP469240RKQ
CSB-BP469240RKQ
CSB-MP469240RKQ
CSB-EP469240RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP469241RKQ
CSB-EP469241RKQ
CSB-BP469241RKQ
CSB-MP469241RKQ
CSB-EP469241RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Protease HtpX homolog (htpX), partial CSB-YP469242RKQ1
CSB-EP469242RKQ1
CSB-BP469242RKQ1
CSB-MP469242RKQ1
CSB-EP469242RKQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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