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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP469423ENW
CSB-EP469423ENW
CSB-BP469423ENW
CSB-MP469423ENW
CSB-EP469423ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L19 (rplS) CSB-YP469424ENW
CSB-EP469424ENW
CSB-BP469424ENW
CSB-MP469424ENW
CSB-EP469424ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Carbon storage regulator (csrA) CSB-YP469425ENW
CSB-EP469425ENW
CSB-BP469425ENW
CSB-MP469425ENW
CSB-EP469425ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DNA mismatch repair protein MutS (mutS), partial CSB-YP469426ENW
CSB-EP469426ENW
CSB-BP469426ENW
CSB-MP469426ENW
CSB-EP469426ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cell division protein FtsB (ftsB), partial CSB-YP469427ENW
CSB-EP469427ENW
CSB-BP469427ENW
CSB-MP469427ENW
CSB-EP469427ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP469428ENW
CSB-EP469428ENW
CSB-BP469428ENW
CSB-MP469428ENW
CSB-EP469428ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein syd (syd) CSB-YP469429ENW
CSB-EP469429ENW
CSB-BP469429ENW
CSB-MP469429ENW
CSB-EP469429ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Deoxyribose-phosphate aldolase (deoC) CSB-YP469430ENW
CSB-EP469430ENW
CSB-BP469430ENW
CSB-MP469430ENW
CSB-EP469430ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP469431ENW
CSB-EP469431ENW
CSB-BP469431ENW
CSB-MP469431ENW
CSB-EP469431ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable phosphoglycerate mutase GpmB (gpmB) CSB-YP469432ENW
CSB-EP469432ENW
CSB-BP469432ENW
CSB-MP469432ENW
CSB-EP469432ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Formyl-coenzyme A transferase (frc) CSB-YP469433ENW
CSB-EP469433ENW
CSB-BP469433ENW
CSB-MP469433ENW
CSB-EP469433ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DNA mismatch repair protein mutH (mutH) CSB-YP469434ENW
CSB-EP469434ENW
CSB-BP469434ENW
CSB-MP469434ENW
CSB-EP469434ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Isopentenyl-diphosphate Delta-isomerase (idi) CSB-YP469435ENW
CSB-EP469435ENW
CSB-BP469435ENW
CSB-MP469435ENW
CSB-EP469435ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli S-adenosylmethionine synthase (metK) CSB-YP469436ENW
CSB-EP469436ENW
CSB-BP469436ENW
CSB-MP469436ENW
CSB-EP469436ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB) CSB-YP469437ENW
CSB-EP469437ENW
CSB-BP469437ENW
CSB-MP469437ENW
CSB-EP469437ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (arnC), partial CSB-YP469438ENW1
CSB-EP469438ENW1
CSB-BP469438ENW1
CSB-MP469438ENW1
CSB-EP469438ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0207 protein yfbR (yfbR) CSB-YP469439ENW
CSB-EP469439ENW
CSB-BP469439ENW
CSB-MP469439ENW
CSB-EP469439ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP469440ENW
CSB-EP469440ENW
CSB-BP469440ENW
CSB-MP469440ENW
CSB-EP469440ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Biotin synthase (bioB) CSB-YP469441ENW
CSB-EP469441ENW
CSB-BP469441ENW
CSB-MP469441ENW
CSB-EP469441ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DNA protection during starvation protein (dps) CSB-YP469442ENW
CSB-EP469442ENW
CSB-BP469442ENW
CSB-MP469442ENW
CSB-EP469442ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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