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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Borrelia burgdorferi Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP470043BUG
CSB-EP470043BUG
CSB-BP470043BUG
CSB-MP470043BUG
CSB-EP470043BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Probable transcriptional regulatory protein BbuZS7_0025 (BbuZS7_0025) CSB-YP470044BUG
CSB-EP470044BUG
CSB-BP470044BUG
CSB-MP470044BUG
CSB-EP470044BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Phosphoglycerate kinase (pgk) CSB-YP470045BUG
CSB-EP470045BUG
CSB-BP470045BUG
CSB-MP470045BUG
CSB-EP470045BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi V-type ATP synthase beta chain (atpB) CSB-YP470046BUG
CSB-EP470046BUG
CSB-BP470046BUG
CSB-MP470046BUG
CSB-EP470046BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Glucosamine-6-phosphate deaminase (nagB) CSB-YP470047BUG
CSB-EP470047BUG
CSB-BP470047BUG
CSB-MP470047BUG
CSB-EP470047BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Flagellar assembly factor FliW (fliW) CSB-YP470048BUG
CSB-EP470048BUG
CSB-BP470048BUG
CSB-MP470048BUG
CSB-EP470048BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Elongation factor P (efp) CSB-YP470049BUG
CSB-EP470049BUG
CSB-BP470049BUG
CSB-MP470049BUG
CSB-EP470049BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Leucine--tRNA ligase (leuS), partial CSB-YP470050BUG
CSB-EP470050BUG
CSB-BP470050BUG
CSB-MP470050BUG
CSB-EP470050BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Enolase (eno) CSB-YP470051BUG
CSB-EP470051BUG
CSB-BP470051BUG
CSB-MP470051BUG
CSB-EP470051BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Tyrosine--tRNA ligase (tyrS) CSB-YP470052BUG
CSB-EP470052BUG
CSB-BP470052BUG
CSB-MP470052BUG
CSB-EP470052BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 30S ribosomal protein S12 (rpsL) CSB-YP470053BUG
CSB-EP470053BUG
CSB-BP470053BUG
CSB-MP470053BUG
CSB-EP470053BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 50S ribosomal protein L10 (rplJ) CSB-YP470054BUG
CSB-EP470054BUG
CSB-BP470054BUG
CSB-MP470054BUG
CSB-EP470054BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Putative 3-methyladenine DNA glycosylase (BbuZS7_0428) CSB-YP470055BUG
CSB-EP470055BUG
CSB-BP470055BUG
CSB-MP470055BUG
CSB-EP470055BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi UvrABC system protein C (uvrC), partial CSB-YP470056BUG
CSB-EP470056BUG
CSB-BP470056BUG
CSB-MP470056BUG
CSB-EP470056BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 50S ribosomal protein L22 (rplV) CSB-YP470057BUG
CSB-EP470057BUG
CSB-BP470057BUG
CSB-MP470057BUG
CSB-EP470057BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 50S ribosomal protein L16 (rplP) CSB-YP470058BUG
CSB-EP470058BUG
CSB-BP470058BUG
CSB-MP470058BUG
CSB-EP470058BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 30S ribosomal protein S8 (rpsH) CSB-YP470059BUG
CSB-EP470059BUG
CSB-BP470059BUG
CSB-MP470059BUG
CSB-EP470059BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 30S ribosomal protein S11 (rpsK) CSB-YP470060BUG
CSB-EP470060BUG
CSB-BP470060BUG
CSB-MP470060BUG
CSB-EP470060BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Methionine--tRNA ligase (metG), partial CSB-YP470061BUG
CSB-EP470061BUG
CSB-BP470061BUG
CSB-MP470061BUG
CSB-EP470061BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Acetate kinase (ackA) CSB-YP470062BUG
CSB-EP470062BUG
CSB-BP470062BUG
CSB-MP470062BUG
CSB-EP470062BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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