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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Vibrio fischeri Non-canonical purine NTP phosphatase (VFMJ11_0572) CSB-YP468563VFC
CSB-EP468563VFC
CSB-BP468563VFC
CSB-MP468563VFC
CSB-EP468563VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP468564VFC
CSB-EP468564VFC
CSB-BP468564VFC
CSB-MP468564VFC
CSB-EP468564VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP468565VFC
CSB-EP468565VFC
CSB-BP468565VFC
CSB-MP468565VFC
CSB-EP468565VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 30S ribosomal protein S21 (rpsU) CSB-YP468566VFC
CSB-EP468566VFC
CSB-BP468566VFC
CSB-MP468566VFC
CSB-EP468566VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP468567VFC
CSB-EP468567VFC
CSB-BP468567VFC
CSB-MP468567VFC
CSB-EP468567VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Leucine--tRNA ligase (leuS), partial CSB-YP468568VFC
CSB-EP468568VFC
CSB-BP468568VFC
CSB-MP468568VFC
CSB-EP468568VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP468569VFC
CSB-EP468569VFC
CSB-BP468569VFC
CSB-MP468569VFC
CSB-EP468569VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Acetylglutamate kinase (argB) CSB-YP468570VFC
CSB-EP468570VFC
CSB-BP468570VFC
CSB-MP468570VFC
CSB-EP468570VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 30S ribosomal protein S6 (rpsF) CSB-YP468571VFC
CSB-EP468571VFC
CSB-BP468571VFC
CSB-MP468571VFC
CSB-EP468571VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP468572VFC
CSB-EP468572VFC
CSB-BP468572VFC
CSB-MP468572VFC
CSB-EP468572VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Adenylate kinase (adk) CSB-YP468573VFC
CSB-EP468573VFC
CSB-BP468573VFC
CSB-MP468573VFC
CSB-EP468573VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP468574VFC
CSB-EP468574VFC
CSB-BP468574VFC
CSB-MP468574VFC
CSB-EP468574VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP468575VFC
CSB-EP468575VFC
CSB-BP468575VFC
CSB-MP468575VFC
CSB-EP468575VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 50S ribosomal protein L11 (rplK) CSB-YP468576VFC
CSB-EP468576VFC
CSB-BP468576VFC
CSB-MP468576VFC
CSB-EP468576VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Fe/S biogenesis protein NfuA (nfuA) CSB-YP468577VFC
CSB-EP468577VFC
CSB-BP468577VFC
CSB-MP468577VFC
CSB-EP468577VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 50S ribosomal protein L25 (rplY) CSB-YP468578VFC
CSB-EP468578VFC
CSB-BP468578VFC
CSB-MP468578VFC
CSB-EP468578VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Serine--tRNA ligase (serS) CSB-YP468579VFC
CSB-EP468579VFC
CSB-BP468579VFC
CSB-MP468579VFC
CSB-EP468579VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri tRNA (mo5U34)-methyltransferase CSB-YP468580VFC
CSB-EP468580VFC
CSB-BP468580VFC
CSB-MP468580VFC
CSB-EP468580VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri ATP synthase gamma chain (atpG) CSB-YP468581VFC
CSB-EP468581VFC
CSB-BP468581VFC
CSB-MP468581VFC
CSB-EP468581VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri ATP phosphoribosyltransferase (hisG) CSB-YP468582VFC
CSB-EP468582VFC
CSB-BP468582VFC
CSB-MP468582VFC
CSB-EP468582VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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