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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodospirillum centenum Ribonuclease Z (rnz) CSB-YP469483RLY
CSB-EP469483RLY
CSB-BP469483RLY
CSB-MP469483RLY
CSB-EP469483RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP469484RLY
CSB-EP469484RLY
CSB-BP469484RLY
CSB-MP469484RLY
CSB-EP469484RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Adenylosuccinate synthetase (purA) CSB-YP469485RLY
CSB-EP469485RLY
CSB-BP469485RLY
CSB-MP469485RLY
CSB-EP469485RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Ketol-acid reductoisomerase (ilvC) CSB-YP469486RLY
CSB-EP469486RLY
CSB-BP469486RLY
CSB-MP469486RLY
CSB-EP469486RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Leucine--tRNA ligase (leuS), partial CSB-YP469487RLY
CSB-EP469487RLY
CSB-BP469487RLY
CSB-MP469487RLY
CSB-EP469487RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Integration host factor subunit beta (ihfB) CSB-YP469488RLY
CSB-EP469488RLY
CSB-BP469488RLY
CSB-MP469488RLY
CSB-EP469488RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum UPF0042 nucleotide-binding protein RC1_2868 (RC1_2868) CSB-YP469489RLY
CSB-EP469489RLY
CSB-BP469489RLY
CSB-MP469489RLY
CSB-EP469489RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum S-adenosylmethionine synthase (metK) CSB-YP469490RLY
CSB-EP469490RLY
CSB-BP469490RLY
CSB-MP469490RLY
CSB-EP469490RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum UPF0060 membrane protein RC1_3291 (RC1_3291), partial CSB-YP469491RLY1
CSB-EP469491RLY1
CSB-BP469491RLY1
CSB-MP469491RLY1
CSB-EP469491RLY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum UPF0234 protein RC1_3464 (RC1_3464) CSB-YP469492RLY
CSB-EP469492RLY
CSB-BP469492RLY
CSB-MP469492RLY
CSB-EP469492RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP469493DXM1
CSB-EP469493DXM1
CSB-BP469493DXM1
CSB-MP469493DXM1
CSB-EP469493DXM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP469494DXM
CSB-EP469494DXM
CSB-BP469494DXM
CSB-MP469494DXM
CSB-EP469494DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Ribosome maturation factor RimP (rimP) CSB-YP469495DXM
CSB-EP469495DXM
CSB-BP469495DXM
CSB-MP469495DXM
CSB-EP469495DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L20 (rplT) CSB-YP469496DXM
CSB-EP469496DXM
CSB-BP469496DXM
CSB-MP469496DXM
CSB-EP469496DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Chaperone protein DnaJ (dnaJ) CSB-YP469497DXM
CSB-EP469497DXM
CSB-BP469497DXM
CSB-MP469497DXM
CSB-EP469497DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii GTPase Der (der) CSB-YP469498DXM
CSB-EP469498DXM
CSB-BP469498DXM
CSB-MP469498DXM
CSB-EP469498DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Outer-membrane lipoprotein carrier protein (lolA) CSB-YP469499DXM
CSB-EP469499DXM
CSB-BP469499DXM
CSB-MP469499DXM
CSB-EP469499DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Chaperone modulatory protein CbpM (cbpM) CSB-YP469500DXM
CSB-EP469500DXM
CSB-BP469500DXM
CSB-MP469500DXM
CSB-EP469500DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L31 (rpmE) CSB-YP469501DXM
CSB-EP469501DXM
CSB-BP469501DXM
CSB-MP469501DXM
CSB-EP469501DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Alanine racemase (alr) CSB-YP469502DXM
CSB-EP469502DXM
CSB-BP469502DXM
CSB-MP469502DXM
CSB-EP469502DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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