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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella paratyphi A Endonuclease 8 (nei) CSB-YP470643SWT
CSB-EP470643SWT
CSB-BP470643SWT
CSB-MP470643SWT
CSB-EP470643SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Pyrimidine-specific ribonucleoside hydrolase RihA (rihA) CSB-YP470644SWT
CSB-EP470644SWT
CSB-BP470644SWT
CSB-MP470644SWT
CSB-EP470644SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Protein CrcB homolog (crcB), partial CSB-YP470645SWT1
CSB-EP470645SWT1
CSB-BP470645SWT1
CSB-MP470645SWT1
CSB-EP470645SWT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP470646SWT
CSB-EP470646SWT
CSB-BP470646SWT
CSB-MP470646SWT
CSB-EP470646SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE (arnE), partial CSB-YP470647SWT1
CSB-EP470647SWT1
CSB-BP470647SWT1
CSB-MP470647SWT1
CSB-EP470647SWT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Nucleoside triphosphatase nudI (nudI) CSB-YP470648SWT
CSB-EP470648SWT
CSB-BP470648SWT
CSB-MP470648SWT
CSB-EP470648SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Bifunctional protein FolD (folD) CSB-YP470649SWT
CSB-EP470649SWT
CSB-BP470649SWT
CSB-MP470649SWT
CSB-EP470649SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Adenylate kinase (adk) CSB-YP470650SWT
CSB-EP470650SWT
CSB-BP470650SWT
CSB-MP470650SWT
CSB-EP470650SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A HMP-PP phosphatase (cof) CSB-YP470651SWT
CSB-EP470651SWT
CSB-BP470651SWT
CSB-MP470651SWT
CSB-EP470651SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP470652SWT
CSB-EP470652SWT
CSB-BP470652SWT
CSB-MP470652SWT
CSB-EP470652SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Anti-adapter protein iraP (iraP) CSB-YP470653SWT
CSB-EP470653SWT
CSB-BP470653SWT
CSB-MP470653SWT
CSB-EP470653SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A 50S ribosomal protein L25 (rplY) CSB-YP470654SWT
CSB-EP470654SWT
CSB-BP470654SWT
CSB-MP470654SWT
CSB-EP470654SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Methionine--tRNA ligase (metG), partial CSB-YP470655SWT
CSB-EP470655SWT
CSB-BP470655SWT
CSB-MP470655SWT
CSB-EP470655SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Carbon storage regulator (csrA) CSB-YP470656SWT
CSB-EP470656SWT
CSB-BP470656SWT
CSB-MP470656SWT
CSB-EP470656SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP470657SWT
CSB-EP470657SWT
CSB-BP470657SWT
CSB-MP470657SWT
CSB-EP470657SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP470658SWT
CSB-EP470658SWT
CSB-BP470658SWT
CSB-MP470658SWT
CSB-EP470658SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A L-fucose isomerase (fucI), partial CSB-YP470659SWT
CSB-EP470659SWT
CSB-BP470659SWT
CSB-MP470659SWT
CSB-EP470659SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Multidrug resistance protein MdtC (mdtC), partial CSB-YP470660SWT
CSB-EP470660SWT
CSB-BP470660SWT
CSB-MP470660SWT
CSB-EP470660SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A DNA mismatch repair protein mutH (mutH) CSB-YP470661SWT
CSB-EP470661SWT
CSB-BP470661SWT
CSB-MP470661SWT
CSB-EP470661SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Peptide chain release factor 2 (prfB) CSB-YP470662SWT
CSB-EP470662SWT
CSB-BP470662SWT
CSB-MP470662SWT
CSB-EP470662SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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