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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptococcus equi subsp. zooepidemicus Regulatory protein recX (recX) CSB-YP470563FMK
CSB-EP470563FMK
CSB-BP470563FMK
CSB-MP470563FMK
CSB-EP470563FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP470564FMK
CSB-EP470564FMK
CSB-BP470564FMK
CSB-MP470564FMK
CSB-EP470564FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Probable manganese-dependent inorganic pyrophosphatase (ppaC) CSB-YP470565FMK
CSB-EP470565FMK
CSB-BP470565FMK
CSB-MP470565FMK
CSB-EP470565FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Probable transcriptional regulatory protein Sez_1681 (Sez_1681) CSB-YP470566FMK
CSB-EP470566FMK
CSB-BP470566FMK
CSB-MP470566FMK
CSB-EP470566FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Trigger factor (tig) CSB-YP470567FMK
CSB-EP470567FMK
CSB-BP470567FMK
CSB-MP470567FMK
CSB-EP470567FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus 50S ribosomal protein L2 (rplB) CSB-YP470568FMK
CSB-EP470568FMK
CSB-BP470568FMK
CSB-MP470568FMK
CSB-EP470568FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus 30S ribosomal protein S17 (rpsQ) CSB-YP470569FMK
CSB-EP470569FMK
CSB-BP470569FMK
CSB-MP470569FMK
CSB-EP470569FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus 30S ribosomal protein S8 (rpsH) CSB-YP470570FMK
CSB-EP470570FMK
CSB-BP470570FMK
CSB-MP470570FMK
CSB-EP470570FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus 50S ribosomal protein L17 (rplQ) CSB-YP470571FMK
CSB-EP470571FMK
CSB-BP470571FMK
CSB-MP470571FMK
CSB-EP470571FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus tRNA (Ile)-lysidine synthase CSB-YP470572FMK
CSB-EP470572FMK
CSB-BP470572FMK
CSB-MP470572FMK
CSB-EP470572FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrogenobaculum sp. UPF0235 protein HY04AAS1_1378 (HY04AAS1_1378) CSB-YP470573HXT
CSB-EP470573HXT
CSB-BP470573HXT
CSB-MP470573HXT
CSB-EP470573HXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrogenobaculum sp. Ribosomal RNA small subunit methyltransferase I (rsmI) CSB-YP470574HXT
CSB-EP470574HXT
CSB-BP470574HXT
CSB-MP470574HXT
CSB-EP470574HXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrogenobaculum sp. 50S ribosomal protein L27 (rpmA) CSB-YP470575HXT
CSB-EP470575HXT
CSB-BP470575HXT
CSB-MP470575HXT
CSB-EP470575HXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrogenobaculum sp. ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP470576HXT
CSB-EP470576HXT
CSB-BP470576HXT
CSB-MP470576HXT
CSB-EP470576HXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrogenobaculum sp. 30S ribosomal protein S3 (rpsC) CSB-YP470577HXT
CSB-EP470577HXT
CSB-BP470577HXT
CSB-MP470577HXT
CSB-EP470577HXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrogenobaculum sp. 30S ribosomal protein S13 (rpsM) CSB-YP470578HXT
CSB-EP470578HXT
CSB-BP470578HXT
CSB-MP470578HXT
CSB-EP470578HXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrogenobaculum sp. tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP470579HXT
CSB-EP470579HXT
CSB-BP470579HXT
CSB-MP470579HXT
CSB-EP470579HXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrogenobaculum sp. ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP470580HXT
CSB-EP470580HXT
CSB-BP470580HXT
CSB-MP470580HXT
CSB-EP470580HXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrogenobaculum sp. Glutamate--tRNA ligase (gltX) CSB-YP470581HXT
CSB-EP470581HXT
CSB-BP470581HXT
CSB-MP470581HXT
CSB-EP470581HXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrogenobaculum sp. NADH-quinone oxidoreductase subunit N 1 (nuoN1), partial CSB-YP470582HXT
CSB-EP470582HXT
CSB-BP470582HXT
CSB-MP470582HXT
CSB-EP470582HXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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