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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus 50S ribosomal protein L14 (rplN) CSB-YP472245BQL
CSB-EP472245BQL
CSB-BP472245BQL
CSB-MP472245BQL
CSB-EP472245BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L18 (rplR) CSB-YP472246BQL
CSB-EP472246BQL
CSB-BP472246BQL
CSB-MP472246BQL
CSB-EP472246BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S11 (rpsK) CSB-YP472247BQL
CSB-EP472247BQL
CSB-BP472247BQL
CSB-MP472247BQL
CSB-EP472247BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methylmalonate semialdehyde dehydrogenase [acylating] (iolA) CSB-YP472248BQL
CSB-EP472248BQL
CSB-BP472248BQL
CSB-MP472248BQL
CSB-EP472248BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Small, acid-soluble spore protein I (sspI) CSB-YP472249BQL
CSB-EP472249BQL
CSB-BP472249BQL
CSB-MP472249BQL
CSB-EP472249BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP472250BQL
CSB-EP472250BQL
CSB-BP472250BQL
CSB-MP472250BQL
CSB-EP472250BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 60 kDa chaperonin (groL), partial CSB-YP472251BQL
CSB-EP472251BQL
CSB-BP472251BQL
CSB-MP472251BQL
CSB-EP472251BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP472252BQL
CSB-EP472252BQL
CSB-BP472252BQL
CSB-MP472252BQL
CSB-EP472252BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Large-conductance mechanosensitive channel (mscL), partial CSB-YP472253BQL1
CSB-EP472253BQL1
CSB-BP472253BQL1
CSB-MP472253BQL1
CSB-EP472253BQL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus S-adenosylmethionine synthase (metK) CSB-YP472254BQL
CSB-EP472254BQL
CSB-BP472254BQL
CSB-MP472254BQL
CSB-EP472254BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP472255BQL
CSB-EP472255BQL
CSB-BP472255BQL
CSB-MP472255BQL
CSB-EP472255BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2-succinylbenzoate--CoA ligase (menE) CSB-YP472256BQL
CSB-EP472256BQL
CSB-BP472256BQL
CSB-MP472256BQL
CSB-EP472256BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Small, acid-soluble spore protein K (sspK) CSB-YP472257BQL
CSB-EP472257BQL
CSB-BP472257BQL
CSB-MP472257BQL
CSB-EP472257BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable cytosol aminopeptidase (pepA) CSB-YP472258BQL
CSB-EP472258BQL
CSB-BP472258BQL
CSB-MP472258BQL
CSB-EP472258BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP472259BQL
CSB-EP472259BQL
CSB-BP472259BQL
CSB-MP472259BQL
CSB-EP472259BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methionine--tRNA ligase (metG), partial CSB-YP472260BQL
CSB-EP472260BQL
CSB-BP472260BQL
CSB-MP472260BQL
CSB-EP472260BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus D-ribose pyranase (rbsD) CSB-YP472261BQL
CSB-EP472261BQL
CSB-BP472261BQL
CSB-MP472261BQL
CSB-EP472261BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP472262BQL1
CSB-EP472262BQL1
CSB-BP472262BQL1
CSB-MP472262BQL1
CSB-EP472262BQL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0342 protein BCAH187_A1043 (BCAH187_A1043) CSB-YP472263BQL
CSB-EP472263BQL
CSB-BP472263BQL
CSB-MP472263BQL
CSB-EP472263BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP472264BQL1
CSB-EP472264BQL1
CSB-BP472264BQL1
CSB-MP472264BQL1
CSB-EP472264BQL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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