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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acidithiobacillus ferrooxidans Chaperone protein DnaK (dnaK), partial CSB-YP472445AWH
CSB-EP472445AWH
CSB-BP472445AWH
CSB-MP472445AWH
CSB-EP472445AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP472446AWH
CSB-EP472446AWH
CSB-BP472446AWH
CSB-MP472446AWH
CSB-EP472446AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L19 (rplS) CSB-YP472447AWH
CSB-EP472447AWH
CSB-BP472447AWH
CSB-MP472447AWH
CSB-EP472447AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP472448AWH
CSB-EP472448AWH
CSB-BP472448AWH
CSB-MP472448AWH
CSB-EP472448AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Iron--sulfur cluster insertion protein ErpA (erpA) CSB-YP472449AWH
CSB-EP472449AWH
CSB-BP472449AWH
CSB-MP472449AWH
CSB-EP472449AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L13 (rplM) CSB-YP472450AWH
CSB-EP472450AWH
CSB-BP472450AWH
CSB-MP472450AWH
CSB-EP472450AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans UPF0178 protein AFE_3267 (AFE_3267) CSB-YP472451AWH
CSB-EP472451AWH
CSB-BP472451AWH
CSB-MP472451AWH
CSB-EP472451AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP472452AWH
CSB-EP472452AWH
CSB-BP472452AWH
CSB-MP472452AWH
CSB-EP472452AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Recombination protein RecR (recR) CSB-YP472453AWH
CSB-EP472453AWH
CSB-BP472453AWH
CSB-MP472453AWH
CSB-EP472453AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP472454AWH
CSB-EP472454AWH
CSB-BP472454AWH
CSB-MP472454AWH
CSB-EP472454AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glycerol kinase (glpK) CSB-YP472455BQM
CSB-EP472455BQM
CSB-BP472455BQM
CSB-MP472455BQM
CSB-EP472455BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP472456BQM
CSB-EP472456BQM
CSB-BP472456BQM
CSB-MP472456BQM
CSB-EP472456BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0271 protein BCAH820_3100 (BCAH820_3100) CSB-YP472457BQM
CSB-EP472457BQM
CSB-BP472457BQM
CSB-MP472457BQM
CSB-EP472457BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Bis (5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] CSB-YP472458BQM
CSB-EP472458BQM
CSB-BP472458BQM
CSB-MP472458BQM
CSB-EP472458BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Indole-3-glycerol phosphate synthase (trpC) CSB-YP472459BQM
CSB-EP472459BQM
CSB-BP472459BQM
CSB-MP472459BQM
CSB-EP472459BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus D-alanine--poly (phosphoribitol) ligase subunit 1, partial CSB-YP472460BQM
CSB-EP472460BQM
CSB-BP472460BQM
CSB-MP472460BQM
CSB-EP472460BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP472461BQM
CSB-EP472461BQM
CSB-BP472461BQM
CSB-MP472461BQM
CSB-EP472461BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0316 protein BCAH820_3389 (BCAH820_3389), partial CSB-YP472462BQM1
CSB-EP472462BQM1
CSB-BP472462BQM1
CSB-MP472462BQM1
CSB-EP472462BQM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP472463BQM
CSB-EP472463BQM
CSB-BP472463BQM
CSB-MP472463BQM
CSB-EP472463BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Uracil phosphoribosyltransferase (upp) CSB-YP472464BQM
CSB-EP472464BQM
CSB-BP472464BQM
CSB-MP472464BQM
CSB-EP472464BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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