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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oryza sativa subsp. japonica Beta-glucosidase 2 (BGLU2) CSB-YP472045OFG
CSB-EP472045OFG
CSB-BP472045OFG
CSB-MP472045OFG
CSB-EP472045OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum Lipoyl synthase 1, mitochondrial (PHATRDRAFT_18029) CSB-YP472046EUF
CSB-EP472046EUF
CSB-BP472046EUF
CSB-MP472046EUF
CSB-EP472046EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum tRNA (guanine (37)-N1)-methyltransferase 2, partial CSB-YP472047EUF
CSB-EP472047EUF
CSB-BP472047EUF
CSB-MP472047EUF
CSB-EP472047EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus UPF0754 membrane protein Aflv_2299 (Aflv_2299), partial CSB-YP472048BZO1
CSB-EP472048BZO1
CSB-BP472048BZO1
CSB-MP472048BZO1
CSB-EP472048BZO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Recombination protein RecR (recR) CSB-YP472049BZO
CSB-EP472049BZO
CSB-BP472049BZO
CSB-MP472049BZO
CSB-EP472049BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP472050BZO
CSB-EP472050BZO
CSB-BP472050BZO
CSB-MP472050BZO
CSB-EP472050BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Alanine racemase (alr) CSB-YP472051BZO
CSB-EP472051BZO
CSB-BP472051BZO
CSB-MP472051BZO
CSB-EP472051BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 10 kDa chaperonin (groS) CSB-YP472052BZO
CSB-EP472052BZO
CSB-BP472052BZO
CSB-MP472052BZO
CSB-EP472052BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Heptaprenylglyceryl phosphate synthase (pcrB) CSB-YP472053BZO
CSB-EP472053BZO
CSB-BP472053BZO
CSB-MP472053BZO
CSB-EP472053BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 30S ribosomal protein S15 (rpsO) CSB-YP472054BZO
CSB-EP472054BZO
CSB-BP472054BZO
CSB-MP472054BZO
CSB-EP472054BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 30S ribosomal protein S16 (rpsP) CSB-YP472055BZO
CSB-EP472055BZO
CSB-BP472055BZO
CSB-MP472055BZO
CSB-EP472055BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP472056BZO
CSB-EP472056BZO
CSB-BP472056BZO
CSB-MP472056BZO
CSB-EP472056BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus UPF0358 protein Aflv_1873 (Aflv_1873) CSB-YP472057BZO
CSB-EP472057BZO
CSB-BP472057BZO
CSB-MP472057BZO
CSB-EP472057BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus UPF0173 metal-dependent hydrolase Aflv_0488 (Aflv_0488) CSB-YP472058BZO
CSB-EP472058BZO
CSB-BP472058BZO
CSB-MP472058BZO
CSB-EP472058BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP472059BZO
CSB-EP472059BZO
CSB-BP472059BZO
CSB-MP472059BZO
CSB-EP472059BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP472060BZO
CSB-EP472060BZO
CSB-BP472060BZO
CSB-MP472060BZO
CSB-EP472060BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Octanoyltransferase LipM (lipM) CSB-YP472061BZO
CSB-EP472061BZO
CSB-BP472061BZO
CSB-MP472061BZO
CSB-EP472061BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP472062BZO
CSB-EP472062BZO
CSB-BP472062BZO
CSB-MP472062BZO
CSB-EP472062BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Tryptophan synthase beta chain (trpB) CSB-YP472063BZO
CSB-EP472063BZO
CSB-BP472063BZO
CSB-MP472063BZO
CSB-EP472063BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Sec-independent protein translocase protein TatA (tatA) CSB-YP472064BZO
CSB-EP472064BZO
CSB-BP472064BZO
CSB-MP472064BZO
CSB-EP472064BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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