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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Klebsiella pneumoniae Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB) CSB-YP471303KBH
CSB-EP471303KBH
CSB-BP471303KBH
CSB-MP471303KBH
CSB-EP471303KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Probable lipid kinase YegS-like (KPK_1632) CSB-YP471304KBH
CSB-EP471304KBH
CSB-BP471304KBH
CSB-MP471304KBH
CSB-EP471304KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP471305KBH
CSB-EP471305KBH
CSB-BP471305KBH
CSB-MP471305KBH
CSB-EP471305KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae D-cysteine desulfhydrase (dcyD) CSB-YP471306KBH
CSB-EP471306KBH
CSB-BP471306KBH
CSB-MP471306KBH
CSB-EP471306KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae tRNA (cmo5U34)-methyltransferase CSB-YP471307KBH
CSB-EP471307KBH
CSB-BP471307KBH
CSB-MP471307KBH
CSB-EP471307KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP471308KBH
CSB-EP471308KBH
CSB-BP471308KBH
CSB-MP471308KBH
CSB-EP471308KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae UPF0266 membrane protein KPK_1957 (KPK_1957) CSB-YP471309KBH
CSB-EP471309KBH
CSB-BP471309KBH
CSB-MP471309KBH
CSB-EP471309KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae UPF0745 protein KPK_1976 (KPK_1976) CSB-YP471310KBH
CSB-EP471310KBH
CSB-BP471310KBH
CSB-MP471310KBH
CSB-EP471310KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae UPF0263 protein KPK_2119 (KPK_2119) CSB-YP471311KBH
CSB-EP471311KBH
CSB-BP471311KBH
CSB-MP471311KBH
CSB-EP471311KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae UPF0061 protein KPK_2154 (KPK_2154) CSB-YP471312KBH
CSB-EP471312KBH
CSB-BP471312KBH
CSB-MP471312KBH
CSB-EP471312KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 4-hydroxy-2-oxovalerate aldolase (mhpE) CSB-YP471313KBH
CSB-EP471313KBH
CSB-BP471313KBH
CSB-MP471313KBH
CSB-EP471313KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae p-hydroxybenzoic acid efflux pump subunit AaeA (aaeA), partial CSB-YP471314KBH1
CSB-EP471314KBH1
CSB-BP471314KBH1
CSB-MP471314KBH1
CSB-EP471314KBH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP471315KBH
CSB-EP471315KBH
CSB-BP471315KBH
CSB-MP471315KBH
CSB-EP471315KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae PKHD-type hydroxylase KPK_3192 (KPK_3192) CSB-YP471316KBH
CSB-EP471316KBH
CSB-BP471316KBH
CSB-MP471316KBH
CSB-EP471316KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Small heat shock protein ibpA (ibpA) CSB-YP471317KBH
CSB-EP471317KBH
CSB-BP471317KBH
CSB-MP471317KBH
CSB-EP471317KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae DNA ligase B (ligB), partial CSB-YP471318KBH
CSB-EP471318KBH
CSB-BP471318KBH
CSB-MP471318KBH
CSB-EP471318KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP471319KBH
CSB-EP471319KBH
CSB-BP471319KBH
CSB-MP471319KBH
CSB-EP471319KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (arnC), partial CSB-YP471320KBH1
CSB-EP471320KBH1
CSB-BP471320KBH1
CSB-MP471320KBH1
CSB-EP471320KBH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase (arnT), partial CSB-YP471321KBH
CSB-BP471321KBH
CSB-MP471321KBH
CSB-EP471321KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Putrescine aminotransferase (patA) CSB-YP471322KBH
CSB-EP471322KBH
CSB-BP471322KBH
CSB-MP471322KBH
CSB-EP471322KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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