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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella schwarzengrund H (+)/Cl (-) exchange transporter ClcA, partial CSB-YP472886SWV
CSB-EP472886SWV
CSB-BP472886SWV
CSB-MP472886SWV
CSB-EP472886SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Glutamyl-tRNA reductase (hemA) CSB-YP472887SWV
CSB-EP472887SWV
CSB-BP472887SWV
CSB-MP472887SWV
CSB-EP472887SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Na (+)/H (+) antiporter nhaB, partial CSB-YP472888SWV
CSB-EP472888SWV
CSB-BP472888SWV
CSB-MP472888SWV
CSB-EP472888SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Uncharacterized Nudix hydrolase NudL (nudL) CSB-YP472889SWV
CSB-EP472889SWV
CSB-BP472889SWV
CSB-MP472889SWV
CSB-EP472889SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Siroheme synthase (cysG) CSB-YP472890SWV
CSB-EP472890SWV
CSB-BP472890SWV
CSB-MP472890SWV
CSB-EP472890SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Thiosulfate sulfurtransferase glpE (glpE) CSB-YP472891SWV
CSB-EP472891SWV
CSB-BP472891SWV
CSB-MP472891SWV
CSB-EP472891SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP472892SWV
CSB-EP472892SWV
CSB-BP472892SWV
CSB-MP472892SWV
CSB-EP472892SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Ribonuclease H (rnhA) CSB-YP472893SWV
CSB-EP472893SWV
CSB-BP472893SWV
CSB-MP472893SWV
CSB-EP472893SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Copper homeostasis protein CutC (cutC) CSB-YP472894SWV
CSB-EP472894SWV
CSB-BP472894SWV
CSB-MP472894SWV
CSB-EP472894SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund C4-dicarboxylate transport protein (dctA), partial CSB-YP472895SWV
CSB-EP472895SWV
CSB-BP472895SWV
CSB-MP472895SWV
CSB-EP472895SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Esterase FrsA (frsA) CSB-YP472896SWV
CSB-EP472896SWV
CSB-BP472896SWV
CSB-MP472896SWV
CSB-EP472896SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0345 protein YaiE (yaiE) CSB-YP472897SWV
CSB-EP472897SWV
CSB-BP472897SWV
CSB-MP472897SWV
CSB-EP472897SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Protein mtfA (mtfA) CSB-YP472898SWV
CSB-EP472898SWV
CSB-BP472898SWV
CSB-MP472898SWV
CSB-EP472898SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP472899SWV
CSB-EP472899SWV
CSB-BP472899SWV
CSB-MP472899SWV
CSB-EP472899SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP472900SWV
CSB-EP472900SWV
CSB-BP472900SWV
CSB-MP472900SWV
CSB-EP472900SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund DNA ligase B (ligB), partial CSB-YP472901SWV
CSB-EP472901SWV
CSB-BP472901SWV
CSB-MP472901SWV
CSB-EP472901SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Leucine--tRNA ligase (leuS), partial CSB-YP472902FMK
CSB-EP472902FMK
CSB-BP472902FMK
CSB-MP472902FMK
CSB-EP472902FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus DNA mismatch repair protein mutL (mutL), partial CSB-YP472903FMK
CSB-EP472903FMK
CSB-BP472903FMK
CSB-MP472903FMK
CSB-EP472903FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus 50S ribosomal protein L34 (rpmH) CSB-YP472904FMK
CSB-EP472904FMK
CSB-BP472904FMK
CSB-MP472904FMK
CSB-EP472904FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Xaa-Pro dipeptidyl-peptidase (pepX), partial CSB-YP472905FMK
CSB-EP472905FMK
CSB-BP472905FMK
CSB-MP472905FMK
CSB-EP472905FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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