Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O157:H7 Sulfite reductase [NADPH] hemoprotein beta-component (cysI) CSB-YP471523EOE
CSB-EP471523EOE
CSB-BP471523EOE
CSB-MP471523EOE
CSB-EP471523EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Aspartate carbamoyltransferase (pyrB) CSB-YP471524EOE
CSB-EP471524EOE
CSB-BP471524EOE
CSB-MP471524EOE
CSB-EP471524EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 tRNA sulfurtransferase (thiI) CSB-YP471525EOE
CSB-EP471525EOE
CSB-BP471525EOE
CSB-MP471525EOE
CSB-EP471525EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0133 protein ybaB (ybaB) CSB-YP471526EOE
CSB-EP471526EOE
CSB-BP471526EOE
CSB-MP471526EOE
CSB-EP471526EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Acetyl esterase (aes) CSB-YP471527EOE
CSB-EP471527EOE
CSB-BP471527EOE
CSB-MP471527EOE
CSB-EP471527EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Spermidine export protein MdtI (mdtI), partial CSB-YP471528EOE1
CSB-EP471528EOE1
CSB-BP471528EOE1
CSB-MP471528EOE1
CSB-EP471528EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Electron transport complex protein RnfD (rnfD), partial CSB-YP471529EOE
CSB-EP471529EOE
CSB-BP471529EOE
CSB-MP471529EOE
CSB-EP471529EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Cysteine desulfuration protein sufE (sufE) CSB-YP471530EOE
CSB-EP471530EOE
CSB-BP471530EOE
CSB-MP471530EOE
CSB-EP471530EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 RNA pyrophosphohydrolase (rppH) CSB-YP471531EOE
CSB-EP471531EOE
CSB-BP471531EOE
CSB-MP471531EOE
CSB-EP471531EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Peptide chain release factor 3 (prfC) CSB-YP471532EOE
CSB-EP471532EOE
CSB-BP471532EOE
CSB-MP471532EOE
CSB-EP471532EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Urease accessory protein UreE (ureE) CSB-YP471533HUX
CSB-EP471533HUX
CSB-BP471533HUX
CSB-MP471533HUX
CSB-EP471533HUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 60 kDa chaperonin (groL), partial CSB-YP471534HUX
CSB-EP471534HUX
CSB-BP471534HUX
CSB-MP471534HUX
CSB-EP471534HUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 3-dehydroquinate dehydratase (aroQ) CSB-YP471535HUX
CSB-EP471535HUX
CSB-BP471535HUX
CSB-MP471535HUX
CSB-EP471535HUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP471536HUX
CSB-EP471536HUX
CSB-BP471536HUX
CSB-MP471536HUX
CSB-EP471536HUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 30S ribosomal protein S21 (rpsU) CSB-YP471537HUX
CSB-EP471537HUX
CSB-BP471537HUX
CSB-MP471537HUX
CSB-EP471537HUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Aspartate--tRNA ligase (aspS), partial CSB-YP471538HUX
CSB-EP471538HUX
CSB-BP471538HUX
CSB-MP471538HUX
CSB-EP471538HUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP471539HUX
CSB-EP471539HUX
CSB-BP471539HUX
CSB-MP471539HUX
CSB-EP471539HUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori GTPase Der (der) CSB-YP471540HUX
CSB-EP471540HUX
CSB-BP471540HUX
CSB-MP471540HUX
CSB-EP471540HUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Recombination protein RecR (recR) CSB-YP471541HUX
CSB-EP471541HUX
CSB-BP471541HUX
CSB-MP471541HUX
CSB-EP471541HUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP471542HUX
CSB-EP471542HUX
CSB-BP471542HUX
CSB-MP471542HUX
CSB-EP471542HUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>