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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O157:H7 H (+)/Cl (-) exchange transporter ClcA, partial CSB-YP471503EOE
CSB-EP471503EOE
CSB-BP471503EOE
CSB-MP471503EOE
CSB-EP471503EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Outer membrane protein assembly factor BamA (bamA) CSB-YP471504EOE
CSB-EP471504EOE
CSB-BP471504EOE
CSB-MP471504EOE
CSB-EP471504EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ribonuclease HII (rnhB) CSB-YP471505EOE
CSB-EP471505EOE
CSB-BP471505EOE
CSB-MP471505EOE
CSB-EP471505EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ribonuclease H (rnhA) CSB-YP471506EOE
CSB-EP471506EOE
CSB-BP471506EOE
CSB-MP471506EOE
CSB-EP471506EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP471507EOE
CSB-EP471507EOE
CSB-BP471507EOE
CSB-MP471507EOE
CSB-EP471507EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP471508EOE
CSB-EP471508EOE
CSB-BP471508EOE
CSB-MP471508EOE
CSB-EP471508EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 HTH-type transcriptional regulator iscR (iscR) CSB-YP471509EOE
CSB-EP471509EOE
CSB-BP471509EOE
CSB-MP471509EOE
CSB-EP471509EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Maltoporin (lamB) CSB-YP471510EOE
CSB-EP471510EOE
CSB-BP471510EOE
CSB-MP471510EOE
CSB-EP471510EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Xanthine phosphoribosyltransferase (gpt) CSB-YP471511EOE
CSB-EP471511EOE
CSB-BP471511EOE
CSB-MP471511EOE
CSB-EP471511EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Probable HTH-type transcriptional regulator matA homolog (matA) CSB-YP471512EOE
CSB-EP471512EOE
CSB-BP471512EOE
CSB-MP471512EOE
CSB-EP471512EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Altronate oxidoreductase (uxaB) CSB-YP471513EOE
CSB-EP471513EOE
CSB-BP471513EOE
CSB-MP471513EOE
CSB-EP471513EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 SsrA-binding protein (smpB) CSB-YP471514EOE
CSB-EP471514EOE
CSB-BP471514EOE
CSB-MP471514EOE
CSB-EP471514EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Divalent-cation tolerance protein CutA (cutA) CSB-YP471515EOE
CSB-EP471515EOE
CSB-BP471515EOE
CSB-MP471515EOE
CSB-EP471515EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 DNA mismatch repair protein mutL (mutL) CSB-YP471516EOE
CSB-EP471516EOE
CSB-BP471516EOE
CSB-MP471516EOE
CSB-EP471516EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 HTH-type transcriptional repressor NsrR (nsrR) CSB-YP471517EOE
CSB-EP471517EOE
CSB-BP471517EOE
CSB-MP471517EOE
CSB-EP471517EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 30S ribosomal protein S6 (rpsF) CSB-YP471518EOE
CSB-EP471518EOE
CSB-BP471518EOE
CSB-MP471518EOE
CSB-EP471518EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Cyanate hydratase (cynS) CSB-YP471519EOE
CSB-EP471519EOE
CSB-BP471519EOE
CSB-MP471519EOE
CSB-EP471519EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 4-hydroxy-2-oxovalerate aldolase (mhpE) CSB-YP471520EOE
CSB-EP471520EOE
CSB-BP471520EOE
CSB-MP471520EOE
CSB-EP471520EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP471521EOE
CSB-EP471521EOE
CSB-BP471521EOE
CSB-MP471521EOE
CSB-EP471521EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 5'/3'-nucleotidase SurE (surE) CSB-YP471522EOE
CSB-EP471522EOE
CSB-BP471522EOE
CSB-MP471522EOE
CSB-EP471522EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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