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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bifidobacterium longum subsp. infantis Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP472105BTB
CSB-EP472105BTB
CSB-BP472105BTB
CSB-MP472105BTB
CSB-EP472105BTB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum subsp. infantis 50S ribosomal protein L31 type B (rpmE2) CSB-YP472106BTB
CSB-EP472106BTB
CSB-BP472106BTB
CSB-MP472106BTB
CSB-EP472106BTB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum subsp. infantis DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP472107BTB
CSB-EP472107BTB
CSB-BP472107BTB
CSB-MP472107BTB
CSB-EP472107BTB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum subsp. infantis Pup--protein ligase (pafA) CSB-YP472108BTB
CSB-EP472108BTB
CSB-BP472108BTB
CSB-MP472108BTB
CSB-EP472108BTB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum subsp. infantis S-adenosylmethionine synthase (metK) CSB-YP472109BTB
CSB-EP472109BTB
CSB-BP472109BTB
CSB-MP472109BTB
CSB-EP472109BTB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP472110AWN
CSB-EP472110AWN
CSB-BP472110AWN
CSB-MP472110AWN
CSB-EP472110AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Chromosomal replication initiator protein DnaA (dnaA) CSB-YP472111AWN
CSB-EP472111AWN
CSB-BP472111AWN
CSB-MP472111AWN
CSB-EP472111AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii UPF0391 membrane protein ABBFA_000052 (ABBFA_000052), partial CSB-YP472112AWN
CSB-EP472112AWN
CSB-BP472112AWN
CSB-MP472112AWN
CSB-EP472112AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP472113AWN
CSB-EP472113AWN
CSB-BP472113AWN
CSB-MP472113AWN
CSB-EP472113AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Probable potassium transport system protein kup (kup), partial CSB-YP472114AWN
CSB-EP472114AWN
CSB-BP472114AWN
CSB-MP472114AWN
CSB-EP472114AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP472115AWN
CSB-EP472115AWN
CSB-BP472115AWN
CSB-MP472115AWN
CSB-EP472115AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP472116AWN
CSB-EP472116AWN
CSB-BP472116AWN
CSB-MP472116AWN
CSB-EP472116AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 50S ribosomal protein L23 (rplW) CSB-YP472117AWN
CSB-EP472117AWN
CSB-BP472117AWN
CSB-MP472117AWN
CSB-EP472117AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 30S ribosomal protein S17 (rpsQ) CSB-YP472118AWN
CSB-EP472118AWN
CSB-BP472118AWN
CSB-MP472118AWN
CSB-EP472118AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 50S ribosomal protein L6 (rplF) CSB-YP472119AWN
CSB-EP472119AWN
CSB-BP472119AWN
CSB-MP472119AWN
CSB-EP472119AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 30S ribosomal protein S13 (rpsM) CSB-YP472120AWN
CSB-EP472120AWN
CSB-BP472120AWN
CSB-MP472120AWN
CSB-EP472120AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Probable rRNA maturation factor (ABBFA_000487) CSB-YP472121AWN
CSB-EP472121AWN
CSB-BP472121AWN
CSB-MP472121AWN
CSB-EP472121AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 50S ribosomal protein L13 (rplM) CSB-YP472122AWN
CSB-EP472122AWN
CSB-BP472122AWN
CSB-MP472122AWN
CSB-EP472122AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Tryptophan synthase alpha chain (trpA) CSB-YP472123AWN
CSB-EP472123AWN
CSB-BP472123AWN
CSB-MP472123AWN
CSB-EP472123AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP472124AWN
CSB-EP472124AWN
CSB-BP472124AWN
CSB-MP472124AWN
CSB-EP472124AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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