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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O157:H7 Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP473826EOE
CSB-EP473826EOE
CSB-BP473826EOE
CSB-MP473826EOE
CSB-EP473826EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Dihydroxy-acid dehydratase (ilvD) CSB-YP473827EOE
CSB-EP473827EOE
CSB-BP473827EOE
CSB-MP473827EOE
CSB-EP473827EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE) CSB-YP473828EOE
CSB-EP473828EOE
CSB-BP473828EOE
CSB-MP473828EOE
CSB-EP473828EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Lipoprotein signal peptidase (lspA), partial CSB-YP473829EOE1
CSB-EP473829EOE1
CSB-BP473829EOE1
CSB-MP473829EOE1
CSB-EP473829EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein fixB (fixB) CSB-YP473830EOE
CSB-EP473830EOE
CSB-BP473830EOE
CSB-MP473830EOE
CSB-EP473830EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Probable intracellular septation protein A (yciB), partial CSB-YP473831EOE1
CSB-EP473831EOE1
CSB-BP473831EOE1
CSB-MP473831EOE1
CSB-EP473831EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 D-tyrosyl-tRNA (Tyr) deacylase CSB-YP473832EOE
CSB-EP473832EOE
CSB-BP473832EOE
CSB-MP473832EOE
CSB-EP473832EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 2-keto-3-deoxygluconate permease (kdgT), partial CSB-YP473833EOE
CSB-EP473833EOE
CSB-BP473833EOE
CSB-MP473833EOE
CSB-EP473833EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Glycerol kinase (glpK) CSB-YP473834EOE
CSB-EP473834EOE
CSB-BP473834EOE
CSB-MP473834EOE
CSB-EP473834EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Glutathione-regulated potassium-efflux system protein kefC (kefC), partial CSB-YP473835EOE
CSB-EP473835EOE
CSB-BP473835EOE
CSB-MP473835EOE
CSB-EP473835EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP473836EOE
CSB-EP473836EOE
CSB-BP473836EOE
CSB-MP473836EOE
CSB-EP473836EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0289 protein yacF (yacF) CSB-YP473837EOE
CSB-EP473837EOE
CSB-BP473837EOE
CSB-MP473837EOE
CSB-EP473837EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Pantothenate synthetase (panC) CSB-YP473838EOE
CSB-EP473838EOE
CSB-BP473838EOE
CSB-MP473838EOE
CSB-EP473838EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0509 protein yciZ (yciZ) CSB-YP473839EOE
CSB-EP473839EOE
CSB-BP473839EOE
CSB-MP473839EOE
CSB-EP473839EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Putative ion-transport protein yfeO (yfeO), partial CSB-YP473840EOE
CSB-EP473840EOE
CSB-BP473840EOE
CSB-MP473840EOE
CSB-EP473840EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein CysZ (cysZ), partial CSB-YP473841EOE1
CSB-EP473841EOE1
CSB-BP473841EOE1
CSB-MP473841EOE1
CSB-EP473841EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ethanolamine ammonia-lyase light chain (eutC) CSB-YP473842EOE
CSB-EP473842EOE
CSB-BP473842EOE
CSB-MP473842EOE
CSB-EP473842EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Met repressor (metJ) CSB-YP473843EOE
CSB-EP473843EOE
CSB-BP473843EOE
CSB-MP473843EOE
CSB-EP473843EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 tRNA (uracil (54)-C (5))-methyltransferase CSB-YP473844EOE
CSB-EP473844EOE
CSB-BP473844EOE
CSB-MP473844EOE
CSB-EP473844EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L7/L12 (rplL) CSB-YP473845EOE
CSB-EP473845EOE
CSB-BP473845EOE
CSB-MP473845EOE
CSB-EP473845EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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