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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Acidithiobacillus ferrooxidans S-adenosylmethionine synthase (metK) CSB-YP473146AWI
CSB-EP473146AWI
CSB-BP473146AWI
CSB-MP473146AWI
CSB-EP473146AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L33 (rpmG) CSB-YP473147AWI
CSB-EP473147AWI
CSB-BP473147AWI
CSB-MP473147AWI
CSB-EP473147AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Thiazole synthase (thiG) CSB-YP473148AWI
CSB-EP473148AWI
CSB-BP473148AWI
CSB-MP473148AWI
CSB-EP473148AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP473149AWI
CSB-EP473149AWI
CSB-BP473149AWI
CSB-MP473149AWI
CSB-EP473149AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Enolase (eno) CSB-YP473150AWI
CSB-EP473150AWI
CSB-BP473150AWI
CSB-MP473150AWI
CSB-EP473150AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Regulatory protein recX (recX) CSB-YP473151AWI
CSB-EP473151AWI
CSB-BP473151AWI
CSB-MP473151AWI
CSB-EP473151AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP473152AWI
CSB-EP473152AWI
CSB-BP473152AWI
CSB-MP473152AWI
CSB-EP473152AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Bifunctional protein GlmU (glmU) CSB-YP473153AWI
CSB-EP473153AWI
CSB-BP473153AWI
CSB-MP473153AWI
CSB-EP473153AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Anthranilate phosphoribosyltransferase (trpD) CSB-YP473154AWI
CSB-EP473154AWI
CSB-BP473154AWI
CSB-MP473154AWI
CSB-EP473154AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP473155AWI
CSB-EP473155AWI
CSB-BP473155AWI
CSB-MP473155AWI
CSB-EP473155AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri D-alanine--D-alanine ligase (ddl) CSB-YP473156VFC
CSB-EP473156VFC
CSB-BP473156VFC
CSB-MP473156VFC
CSB-EP473156VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 8-amino-7-oxononanoate synthase (bioF) CSB-YP473157VFC
CSB-EP473157VFC
CSB-BP473157VFC
CSB-MP473157VFC
CSB-EP473157VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Hydroxylamine reductase (hcp), partial CSB-YP473158VFC
CSB-EP473158VFC
CSB-BP473158VFC
CSB-MP473158VFC
CSB-EP473158VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Gamma-glutamyl phosphate reductase (proA) CSB-YP473159SWK
CSB-EP473159SWK
CSB-BP473159SWK
CSB-MP473159SWK
CSB-EP473159SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Histidinol-phosphate aminotransferase (hisC) CSB-YP473160SWK
CSB-EP473160SWK
CSB-BP473160SWK
CSB-MP473160SWK
CSB-EP473160SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP473161SWK
CSB-EP473161SWK
CSB-BP473161SWK
CSB-MP473161SWK
CSB-EP473161SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona UPF0758 protein yicR (yicR) CSB-YP473162SWK
CSB-EP473162SWK
CSB-BP473162SWK
CSB-MP473162SWK
CSB-EP473162SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Ribonuclease PH (rph) CSB-YP473163SWK
CSB-EP473163SWK
CSB-BP473163SWK
CSB-MP473163SWK
CSB-EP473163SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 2-aminoethylphosphonate--pyruvate transaminase (phnW) CSB-YP473164SWK
CSB-EP473164SWK
CSB-BP473164SWK
CSB-MP473164SWK
CSB-EP473164SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 50S ribosomal protein L36 (rpmJ) CSB-YP473165SWK
CSB-EP473165SWK
CSB-BP473165SWK
CSB-MP473165SWK
CSB-EP473165SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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