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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptococcus equi subsp. zooepidemicus 30S ribosomal protein S18 (rpsR) CSB-YP472906FMK
CSB-EP472906FMK
CSB-BP472906FMK
CSB-MP472906FMK
CSB-EP472906FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Heat-inducible transcription repressor HrcA (hrcA) CSB-YP472907FMK
CSB-EP472907FMK
CSB-BP472907FMK
CSB-MP472907FMK
CSB-EP472907FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Ribosome-binding factor A (rbfA) CSB-YP472908FMK
CSB-EP472908FMK
CSB-BP472908FMK
CSB-MP472908FMK
CSB-EP472908FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP472909FMK
CSB-EP472909FMK
CSB-BP472909FMK
CSB-MP472909FMK
CSB-EP472909FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Uridine kinase (udk) CSB-YP472910FMK
CSB-EP472910FMK
CSB-BP472910FMK
CSB-MP472910FMK
CSB-EP472910FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus UPF0042 nucleotide-binding protein Sez_0730 (Sez_0730) CSB-YP472911FMK
CSB-EP472911FMK
CSB-BP472911FMK
CSB-MP472911FMK
CSB-EP472911FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus 50S ribosomal protein L31 type B (rpmE2) CSB-YP472912FMK
CSB-EP472912FMK
CSB-BP472912FMK
CSB-MP472912FMK
CSB-EP472912FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus DNA ligase (ligA), partial CSB-YP472913FMK
CSB-EP472913FMK
CSB-BP472913FMK
CSB-MP472913FMK
CSB-EP472913FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus ATP synthase epsilon chain (atpC) CSB-YP472914FMK
CSB-EP472914FMK
CSB-BP472914FMK
CSB-MP472914FMK
CSB-EP472914FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus 3-dehydroquinate dehydratase (aroD) CSB-YP472915FMK
CSB-EP472915FMK
CSB-BP472915FMK
CSB-MP472915FMK
CSB-EP472915FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Deoxyribose-phosphate aldolase (deoC) CSB-YP472916FMK
CSB-EP472916FMK
CSB-BP472916FMK
CSB-MP472916FMK
CSB-EP472916FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Probable 2- (5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase CSB-YP472917FMK
CSB-EP472917FMK
CSB-BP472917FMK
CSB-MP472917FMK
CSB-EP472917FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus 50S ribosomal protein L21 (rplU) CSB-YP472918FMK
CSB-EP472918FMK
CSB-BP472918FMK
CSB-MP472918FMK
CSB-EP472918FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP472919FMK
CSB-EP472919FMK
CSB-BP472919FMK
CSB-MP472919FMK
CSB-EP472919FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Elongation factor 4 (lepA), partial CSB-YP472920FMK
CSB-EP472920FMK
CSB-BP472920FMK
CSB-MP472920FMK
CSB-EP472920FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus SsrA-binding protein (smpB) CSB-YP472921FMK
CSB-EP472921FMK
CSB-BP472921FMK
CSB-MP472921FMK
CSB-EP472921FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Holliday junction resolvase recU (recU) CSB-YP472922FMK
CSB-EP472922FMK
CSB-BP472922FMK
CSB-MP472922FMK
CSB-EP472922FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Uracil phosphoribosyltransferase (upp) CSB-YP472923FMK
CSB-EP472923FMK
CSB-BP472923FMK
CSB-MP472923FMK
CSB-EP472923FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Serine/threonine transporter sstT (sstT), partial CSB-YP472924FMK
CSB-EP472924FMK
CSB-BP472924FMK
CSB-MP472924FMK
CSB-EP472924FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus 30S ribosomal protein S10 (rpsJ) CSB-YP472925FMK
CSB-EP472925FMK
CSB-BP472925FMK
CSB-MP472925FMK
CSB-EP472925FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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