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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP474086ENW
CSB-EP474086ENW
CSB-BP474086ENW
CSB-MP474086ENW
CSB-EP474086ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein-export protein secB (secB) CSB-YP474087ENW
CSB-EP474087ENW
CSB-BP474087ENW
CSB-MP474087ENW
CSB-EP474087ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP474088ENW
CSB-EP474088ENW
CSB-BP474088ENW
CSB-MP474088ENW
CSB-EP474088ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Small heat shock protein ibpB (ibpB) CSB-YP474089ENW
CSB-EP474089ENW
CSB-BP474089ENW
CSB-MP474089ENW
CSB-EP474089ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Membrane protein insertase YidC (yidC), partial CSB-YP474090ENW1
CSB-EP474090ENW1
CSB-BP474090ENW1
CSB-MP474090ENW1
CSB-EP474090ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP474091ENW
CSB-EP474091ENW
CSB-BP474091ENW
CSB-MP474091ENW
CSB-EP474091ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP474092ENW
CSB-EP474092ENW
CSB-BP474092ENW
CSB-MP474092ENW
CSB-EP474092ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Bifunctional protein HldE (hldE) CSB-YP474093ENW
CSB-EP474093ENW
CSB-BP474093ENW
CSB-MP474093ENW
CSB-EP474093ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli G/U mismatch-specific DNA glycosylase (mug) CSB-YP474094ENW
CSB-EP474094ENW
CSB-BP474094ENW
CSB-MP474094ENW
CSB-EP474094ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ketol-acid reductoisomerase (ilvC) CSB-YP474095ENW
CSB-EP474095ENW
CSB-BP474095ENW
CSB-MP474095ENW
CSB-EP474095ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein CyaY (cyaY) CSB-YP474096ENW
CSB-EP474096ENW
CSB-BP474096ENW
CSB-MP474096ENW
CSB-EP474096ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein FdhD (fdhD) CSB-YP474097ENW
CSB-EP474097ENW
CSB-BP474097ENW
CSB-MP474097ENW
CSB-EP474097ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli L-rhamnose-proton symporter (rhaT), partial CSB-YP474098ENW
CSB-EP474098ENW
CSB-BP474098ENW
CSB-MP474098ENW
CSB-EP474098ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Met repressor (metJ) CSB-YP474099ENW
CSB-EP474099ENW
CSB-BP474099ENW
CSB-MP474099ENW
CSB-EP474099ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0115 protein yfcN (yfcN) CSB-YP474100ENW
CSB-EP474100ENW
CSB-BP474100ENW
CSB-MP474100ENW
CSB-EP474100ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP474101ENW
CSB-EP474101ENW
CSB-BP474101ENW
CSB-MP474101ENW
CSB-EP474101ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP474102ENW
CSB-EP474102ENW
CSB-BP474102ENW
CSB-MP474102ENW
CSB-EP474102ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli HTH-type transcriptional regulator iscR (iscR) CSB-YP474103ENW
CSB-EP474103ENW
CSB-BP474103ENW
CSB-MP474103ENW
CSB-EP474103ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP474104ENW
CSB-EP474104ENW
CSB-BP474104ENW
CSB-MP474104ENW
CSB-EP474104ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Autonomous glycyl radical cofactor (grcA) CSB-YP474105ENW
CSB-EP474105ENW
CSB-BP474105ENW
CSB-MP474105ENW
CSB-EP474105ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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