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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Helicobacter pylori Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP474286HUY
CSB-EP474286HUY
CSB-BP474286HUY
CSB-MP474286HUY
CSB-EP474286HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP474287HUY
CSB-EP474287HUY
CSB-BP474287HUY
CSB-MP474287HUY
CSB-EP474287HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP474288HUY
CSB-EP474288HUY
CSB-BP474288HUY
CSB-MP474288HUY
CSB-EP474288HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Urease accessory protein UreF (ureF) CSB-YP474289HUY
CSB-EP474289HUY
CSB-BP474289HUY
CSB-MP474289HUY
CSB-EP474289HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 30S ribosomal protein S9 (rpsI) CSB-YP474290HUY
CSB-EP474290HUY
CSB-BP474290HUY
CSB-MP474290HUY
CSB-EP474290HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 50S ribosomal protein L35 (rpmI) CSB-YP474291HUY
CSB-EP474291HUY
CSB-BP474291HUY
CSB-MP474291HUY
CSB-EP474291HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Peptide chain release factor 2 (prfB) CSB-YP474292HUY
CSB-EP474292HUY
CSB-BP474292HUY
CSB-MP474292HUY
CSB-EP474292HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Adenylosuccinate synthetase (SJAG_00044) CSB-YP474293SXT
CSB-EP474293SXT
CSB-BP474293SXT
CSB-MP474293SXT
CSB-EP474293SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus U3 small nucleolar RNA-associated protein 25 (utp25), partial CSB-YP474294SXT
CSB-EP474294SXT
CSB-BP474294SXT
CSB-MP474294SXT
CSB-EP474294SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Probable cytosolic Fe-S cluster assembly factor SJAG_02895 (SJAG_02895), partial CSB-YP474295SXT
CSB-EP474295SXT
CSB-BP474295SXT
CSB-MP474295SXT
CSB-EP474295SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Altered inheritance of mitochondria protein 31, mitochondrial (aim31), partial CSB-YP474296SXT1
CSB-EP474296SXT1
CSB-BP474296SXT1
CSB-MP474296SXT1
CSB-EP474296SXT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Translation factor guf1, mitochondrial (guf1), partial CSB-YP474297SXT
CSB-EP474297SXT
CSB-BP474297SXT
CSB-MP474297SXT
CSB-EP474297SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Branchiostoma floridae UPF0728 protein (BRAFLDRAFT_270831) CSB-YP474298BVY
CSB-EP474298BVY
CSB-BP474298BVY
CSB-MP474298BVY
CSB-EP474298BVY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Branchiostoma floridae Putative deoxyribonucleoside 5'-monophosphate N-glycosidase CSB-YP474299BVY
CSB-EP474299BVY
CSB-BP474299BVY
CSB-MP474299BVY
CSB-EP474299BVY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei ATPase get3 (get3) CSB-YP474300ETM
CSB-EP474300ETM
CSB-BP474300ETM
CSB-MP474300ETM
CSB-EP474300ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Signal peptidase complex catalytic subunit sec11 (sec11), partial CSB-YP474301ETM
CSB-EP474301ETM
CSB-BP474301ETM
CSB-MP474301ETM
CSB-EP474301ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Carboxypeptidase Y homolog A (cpyA) CSB-YP474302ETM
CSB-EP474302ETM
CSB-BP474302ETM
CSB-MP474302ETM
CSB-EP474302ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Methionine aminopeptidase 2 homolog PMAA_077770 (PMAA_077770) CSB-YP474303ETM
CSB-EP474303ETM
CSB-BP474303ETM
CSB-MP474303ETM
CSB-EP474303ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei E3 ubiquitin ligase complex SCF subunit sconC (sconC) CSB-YP474304ETM
CSB-EP474304ETM
CSB-BP474304ETM
CSB-MP474304ETM
CSB-EP474304ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Restriction of telomere capping protein 5 (rtc5), partial CSB-YP474305ETM
CSB-EP474305ETM
CSB-BP474305ETM
CSB-MP474305ETM
CSB-EP474305ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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