Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Penicillium marneffei Cytosolic Fe-S cluster assembly factor nar1 (nar1), partial CSB-YP474306ETM
CSB-EP474306ETM
CSB-BP474306ETM
CSB-MP474306ETM
CSB-EP474306ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 50S ribosomal protein L21 (rplU1) CSB-YP474307AZX
CSB-EP474307AZX
CSB-BP474307AZX
CSB-MP474307AZX
CSB-EP474307AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Serine--tRNA ligase (serS) CSB-YP474308AZX
CSB-EP474308AZX
CSB-BP474308AZX
CSB-MP474308AZX
CSB-EP474308AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 50S ribosomal protein L29 (rpmC) CSB-YP474309AZX
CSB-EP474309AZX
CSB-BP474309AZX
CSB-MP474309AZX
CSB-EP474309AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii tRNA (cmo5U34)-methyltransferase CSB-YP474310AZX
CSB-EP474310AZX
CSB-BP474310AZX
CSB-MP474310AZX
CSB-EP474310AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Bifunctional protein GlmU (glmU) CSB-YP474311AZX
CSB-EP474311AZX
CSB-BP474311AZX
CSB-MP474311AZX
CSB-EP474311AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Alanine racemase (alr) CSB-YP474312AZX
CSB-EP474312AZX
CSB-BP474312AZX
CSB-MP474312AZX
CSB-EP474312AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Orotate phosphoribosyltransferase (pyrE1) CSB-YP474313AZX
CSB-EP474313AZX
CSB-BP474313AZX
CSB-MP474313AZX
CSB-EP474313AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Phosphopantetheine adenylyltransferase (coaD) CSB-YP474314AZX
CSB-EP474314AZX
CSB-BP474314AZX
CSB-MP474314AZX
CSB-EP474314AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP474315AZX
CSB-EP474315AZX
CSB-BP474315AZX
CSB-MP474315AZX
CSB-EP474315AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Undecaprenyl-diphosphatase (uppP), partial CSB-YP474316EYX1
CSB-EP474316EYX1
CSB-BP474316EYX1
CSB-MP474316EYX1
CSB-EP474316EYX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP474317EYX
CSB-EP474317EYX
CSB-BP474317EYX
CSB-MP474317EYX
CSB-EP474317EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Regulatory protein recX (recX) CSB-YP474318EYX
CSB-EP474318EYX
CSB-BP474318EYX
CSB-MP474318EYX
CSB-EP474318EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii UPF0747 protein Paes_1842 (Paes_1842), partial CSB-YP474319EYX
CSB-EP474319EYX
CSB-BP474319EYX
CSB-MP474319EYX
CSB-EP474319EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP474320EYX
CSB-EP474320EYX
CSB-BP474320EYX
CSB-MP474320EYX
CSB-EP474320EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii UPF0235 protein Paes_1868 (Paes_1868) CSB-YP474321EYX
CSB-EP474321EYX
CSB-BP474321EYX
CSB-MP474321EYX
CSB-EP474321EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Ribosome maturation factor RimP (rimP) CSB-YP474322EYX
CSB-EP474322EYX
CSB-BP474322EYX
CSB-MP474322EYX
CSB-EP474322EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP474323EYX
CSB-EP474323EYX
CSB-BP474323EYX
CSB-MP474323EYX
CSB-EP474323EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii 30S ribosomal protein S4 (rpsD) CSB-YP474324EYX
CSB-EP474324EYX
CSB-BP474324EYX
CSB-MP474324EYX
CSB-EP474324EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii 50S ribosomal protein L30 (rpmD) CSB-YP474325EYX
CSB-EP474325EYX
CSB-BP474325EYX
CSB-MP474325EYX
CSB-EP474325EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>