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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Geobacter bemidjiensis Dihydroorotase (pyrC) CSB-YP473106GFN
CSB-EP473106GFN
CSB-BP473106GFN
CSB-MP473106GFN
CSB-EP473106GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Alanine racemase (alr) CSB-YP473107GFN
CSB-EP473107GFN
CSB-BP473107GFN
CSB-MP473107GFN
CSB-EP473107GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP473108GFN
CSB-EP473108GFN
CSB-BP473108GFN
CSB-MP473108GFN
CSB-EP473108GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 50S ribosomal protein L35 (rpmI) CSB-YP473109GFN
CSB-EP473109GFN
CSB-BP473109GFN
CSB-MP473109GFN
CSB-EP473109GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP473110GFN
CSB-EP473110GFN
CSB-BP473110GFN
CSB-MP473110GFN
CSB-EP473110GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis CinA-like protein (Gbem_3628) CSB-YP473111GFN
CSB-EP473111GFN
CSB-BP473111GFN
CSB-MP473111GFN
CSB-EP473111GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP473112GFN
CSB-EP473112GFN
CSB-BP473112GFN
CSB-MP473112GFN
CSB-EP473112GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP473113GFN
CSB-EP473113GFN
CSB-BP473113GFN
CSB-MP473113GFN
CSB-EP473113GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis ATP phosphoribosyltransferase (hisG) CSB-YP473114GFN
CSB-EP473114GFN
CSB-BP473114GFN
CSB-MP473114GFN
CSB-EP473114GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis GTPase Der (der) CSB-YP473115GFN
CSB-EP473115GFN
CSB-BP473115GFN
CSB-MP473115GFN
CSB-EP473115GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP473116GFN
CSB-EP473116GFN
CSB-BP473116GFN
CSB-MP473116GFN
CSB-EP473116GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP473117GFN
CSB-EP473117GFN
CSB-BP473117GFN
CSB-MP473117GFN
CSB-EP473117GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis NADH-quinone oxidoreductase subunit K 2 (nuoK2), partial CSB-YP473118GFN1
CSB-EP473118GFN1
CSB-BP473118GFN1
CSB-MP473118GFN1
CSB-EP473118GFN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis ATP synthase subunit c (atpE), partial CSB-YP473119GFN1
CSB-EP473119GFN1
CSB-BP473119GFN1
CSB-MP473119GFN1
CSB-EP473119GFN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP473120GFN
CSB-EP473120GFN
CSB-BP473120GFN
CSB-MP473120GFN
CSB-EP473120GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 30S ribosomal protein S12 (rpsL) CSB-YP473121GFN
CSB-EP473121GFN
CSB-BP473121GFN
CSB-MP473121GFN
CSB-EP473121GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 30S ribosomal protein S19 (rpsS) CSB-YP473122GFN
CSB-EP473122GFN
CSB-BP473122GFN
CSB-MP473122GFN
CSB-EP473122GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 50S ribosomal protein L29 (rpmC) CSB-YP473123GFN
CSB-EP473123GFN
CSB-BP473123GFN
CSB-MP473123GFN
CSB-EP473123GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 50S ribosomal protein L36 (rpmJ) CSB-YP473124GFN
CSB-EP473124GFN
CSB-BP473124GFN
CSB-MP473124GFN
CSB-EP473124GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Sugar fermentation stimulation protein homolog (sfsA) CSB-YP473125GFN
CSB-EP473125GFN
CSB-BP473125GFN
CSB-MP473125GFN
CSB-EP473125GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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