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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Anaeromyxobacter sp. UvrABC system protein C (uvrC), partial CSB-YP474706BYT
CSB-EP474706BYT
CSB-BP474706BYT
CSB-MP474706BYT
CSB-EP474706BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. UPF0235 protein AnaeK_1146 (AnaeK_1146) CSB-YP474707BYT
CSB-EP474707BYT
CSB-BP474707BYT
CSB-MP474707BYT
CSB-EP474707BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. V-type ATP synthase subunit D (atpD) CSB-YP474708BYT
CSB-EP474708BYT
CSB-BP474708BYT
CSB-MP474708BYT
CSB-EP474708BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Dihydrodipicolinate reductase (dapB) CSB-YP474709BYT
CSB-EP474709BYT
CSB-BP474709BYT
CSB-MP474709BYT
CSB-EP474709BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP474710BYT
CSB-EP474710BYT
CSB-BP474710BYT
CSB-MP474710BYT
CSB-EP474710BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Dihydroxy-acid dehydratase (ilvD), partial CSB-YP474711BYT
CSB-EP474711BYT
CSB-BP474711BYT
CSB-MP474711BYT
CSB-EP474711BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP474712BYT
CSB-EP474712BYT
CSB-BP474712BYT
CSB-MP474712BYT
CSB-EP474712BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Phosphate acyltransferase (plsX) CSB-YP474713BYT
CSB-EP474713BYT
CSB-BP474713BYT
CSB-MP474713BYT
CSB-EP474713BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 50S ribosomal protein L21 (rplU) CSB-YP474714BYT
CSB-EP474714BYT
CSB-BP474714BYT
CSB-MP474714BYT
CSB-EP474714BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP474715BYT1
CSB-EP474715BYT1
CSB-BP474715BYT1
CSB-MP474715BYT1
CSB-EP474715BYT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Putative 3-methyladenine DNA glycosylase (AnaeK_4364) CSB-YP474716BYT
CSB-EP474716BYT
CSB-BP474716BYT
CSB-MP474716BYT
CSB-EP474716BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP474717BYT
CSB-EP474717BYT
CSB-BP474717BYT
CSB-MP474717BYT
CSB-EP474717BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP474718BYT
CSB-EP474718BYT
CSB-BP474718BYT
CSB-MP474718BYT
CSB-EP474718BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP474719BYT
CSB-EP474719BYT
CSB-BP474719BYT
CSB-MP474719BYT
CSB-EP474719BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Shikimate dehydrogenase (aroE) CSB-YP474720BYT
CSB-EP474720BYT
CSB-BP474720BYT
CSB-MP474720BYT
CSB-EP474720BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. C4-dicarboxylate transport protein (dctA), partial CSB-YP474721BYT
CSB-EP474721BYT
CSB-BP474721BYT
CSB-MP474721BYT
CSB-EP474721BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Suppressor of hydroxyurea sensitivity protein 2 (SHU2) CSB-YP474722KBK
CSB-EP474722KBK
CSB-BP474722KBK
CSB-MP474722KBK
CSB-EP474722KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Matrix protein 1 (M) CSB-YP474723IMT
CSB-EP474723IMT
CSB-BP474723IMT
CSB-MP474723IMT
CSB-EP474723IMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Protein PB1-F2 (PB1-F2) CSB-YP474724IMT
CSB-EP474724IMT
CSB-BP474724IMT
CSB-MP474724IMT
CSB-EP474724IMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrovipera lebetina C-type lectin A13 CSB-YP474725MPJ
CSB-EP474725MPJ
CSB-BP474725MPJ
CSB-MP474725MPJ
CSB-EP474725MPJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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