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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella agona Adenylate kinase (adk) CSB-YP473166SWK
CSB-EP473166SWK
CSB-BP473166SWK
CSB-MP473166SWK
CSB-EP473166SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Multidrug resistance protein MdtB (mdtB), partial CSB-YP473167SWK
CSB-EP473167SWK
CSB-BP473167SWK
CSB-MP473167SWK
CSB-EP473167SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Chaperone protein TorD (torD) CSB-YP473168SWK
CSB-EP473168SWK
CSB-BP473168SWK
CSB-MP473168SWK
CSB-EP473168SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Bifunctional protein FolD (folD) CSB-YP473169SWK
CSB-EP473169SWK
CSB-BP473169SWK
CSB-MP473169SWK
CSB-EP473169SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP473170SWK
CSB-EP473170SWK
CSB-BP473170SWK
CSB-MP473170SWK
CSB-EP473170SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona HTH-type transcriptional regulator yidZ (yidZ) CSB-YP473171SWK
CSB-EP473171SWK
CSB-BP473171SWK
CSB-MP473171SWK
CSB-EP473171SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona ATP synthase gamma chain (atpG) CSB-YP473172SWK
CSB-EP473172SWK
CSB-BP473172SWK
CSB-MP473172SWK
CSB-EP473172SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA) CSB-YP473173SWK
CSB-EP473173SWK
CSB-BP473173SWK
CSB-MP473173SWK
CSB-EP473173SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Protein CyaY (cyaY) CSB-YP473174SWK
CSB-EP473174SWK
CSB-BP473174SWK
CSB-MP473174SWK
CSB-EP473174SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Probable rRNA maturation factor YbeY (ybeY) CSB-YP473175SWK
CSB-EP473175SWK
CSB-BP473175SWK
CSB-MP473175SWK
CSB-EP473175SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Potassium-transporting ATPase B chain (kdpB), partial CSB-YP473176SWK1
CSB-EP473176SWK1
CSB-BP473176SWK1
CSB-MP473176SWK1
CSB-EP473176SWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE (arnE), partial CSB-YP473177SWK1
CSB-EP473177SWK1
CSB-BP473177SWK1
CSB-MP473177SWK1
CSB-EP473177SWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP473178SWK
CSB-EP473178SWK
CSB-BP473178SWK
CSB-MP473178SWK
CSB-EP473178SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona UPF0207 protein yfbR (yfbR) CSB-YP473179SWK
CSB-EP473179SWK
CSB-BP473179SWK
CSB-MP473179SWK
CSB-EP473179SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP473180SWK
CSB-EP473180SWK
CSB-BP473180SWK
CSB-MP473180SWK
CSB-EP473180SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona D-tyrosyl-tRNA (Tyr) deacylase CSB-YP473181SWK
CSB-EP473181SWK
CSB-BP473181SWK
CSB-MP473181SWK
CSB-EP473181SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona UvrABC system protein B (uvrB), partial CSB-YP473182SWK
CSB-EP473182SWK
CSB-BP473182SWK
CSB-MP473182SWK
CSB-EP473182SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Glutamate--tRNA ligase (gltX) CSB-YP473183SWK
CSB-EP473183SWK
CSB-BP473183SWK
CSB-MP473183SWK
CSB-EP473183SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona L-rhamnose isomerase (rhaA) CSB-YP473184SWK
CSB-EP473184SWK
CSB-BP473184SWK
CSB-MP473184SWK
CSB-EP473184SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Probable fructose-6-phosphate aldolase (fsa) CSB-YP473185SWK
CSB-EP473185SWK
CSB-BP473185SWK
CSB-MP473185SWK
CSB-EP473185SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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