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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Vibrio fischeri Glutamine--tRNA ligase (glnS) CSB-YP475006VFC
CSB-EP475006VFC
CSB-BP475006VFC
CSB-MP475006VFC
CSB-EP475006VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri LexA repressor (lexA) CSB-YP475007VFC
CSB-EP475007VFC
CSB-BP475007VFC
CSB-MP475007VFC
CSB-EP475007VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP475008VFC
CSB-EP475008VFC
CSB-BP475008VFC
CSB-MP475008VFC
CSB-EP475008VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Electron transport complex protein RnfG (rnfG), partial CSB-YP475009VFC1
CSB-EP475009VFC1
CSB-BP475009VFC1
CSB-MP475009VFC1
CSB-EP475009VFC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP475010VFC
CSB-EP475010VFC
CSB-BP475010VFC
CSB-MP475010VFC
CSB-EP475010VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Bifunctional protein GlmU (glmU) CSB-YP475011VFC
CSB-EP475011VFC
CSB-BP475011VFC
CSB-MP475011VFC
CSB-EP475011VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP475012VFC
CSB-EP475012VFC
CSB-BP475012VFC
CSB-MP475012VFC
CSB-EP475012VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP475013VFC
CSB-EP475013VFC
CSB-BP475013VFC
CSB-MP475013VFC
CSB-EP475013VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP475014VFC
CSB-EP475014VFC
CSB-BP475014VFC
CSB-MP475014VFC
CSB-EP475014VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri D-ribose pyranase (rbsD) CSB-YP475015VFC
CSB-EP475015VFC
CSB-BP475015VFC
CSB-MP475015VFC
CSB-EP475015VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Sulfurtransferase TusA homolog (tusA) CSB-YP475016VFC
CSB-EP475016VFC
CSB-BP475016VFC
CSB-MP475016VFC
CSB-EP475016VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA) CSB-YP475017VFC
CSB-EP475017VFC
CSB-BP475017VFC
CSB-MP475017VFC
CSB-EP475017VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri D-tyrosyl-tRNA (Tyr) deacylase CSB-YP475018VFC
CSB-EP475018VFC
CSB-BP475018VFC
CSB-MP475018VFC
CSB-EP475018VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Nucleoid occlusion factor SlmA (slmA) CSB-YP475019VFC
CSB-EP475019VFC
CSB-BP475019VFC
CSB-MP475019VFC
CSB-EP475019VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri o-succinylbenzoate synthase (menC) CSB-YP475020VFC
CSB-EP475020VFC
CSB-BP475020VFC
CSB-MP475020VFC
CSB-EP475020VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Probable septum site-determining protein MinC (minC) CSB-YP475021VFC
CSB-EP475021VFC
CSB-BP475021VFC
CSB-MP475021VFC
CSB-EP475021VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 50S ribosomal protein L22 (rplV) CSB-YP475022VFC
CSB-EP475022VFC
CSB-BP475022VFC
CSB-MP475022VFC
CSB-EP475022VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP475023VFC
CSB-EP475023VFC
CSB-BP475023VFC
CSB-MP475023VFC
CSB-EP475023VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri UPF0061 protein VFMJ11_1896 (VFMJ11_1896) CSB-YP475024VFC
CSB-EP475024VFC
CSB-BP475024VFC
CSB-MP475024VFC
CSB-EP475024VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Chorismate synthase (aroC) CSB-YP475025VFC
CSB-EP475025VFC
CSB-BP475025VFC
CSB-MP475025VFC
CSB-EP475025VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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