Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Endonuclease 8 (nei) CSB-YP474126ENW
CSB-EP474126ENW
CSB-BP474126ENW
CSB-MP474126ENW
CSB-EP474126ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative transport protein YbjL (ybjL), partial CSB-YP474127ENW
CSB-EP474127ENW
CSB-BP474127ENW
CSB-MP474127ENW
CSB-EP474127ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable endonuclease 4 (nfo) CSB-YP474128ENW
CSB-EP474128ENW
CSB-BP474128ENW
CSB-MP474128ENW
CSB-EP474128ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Vitamin B12 import ATP-binding protein BtuD (btuD) CSB-YP474129ENW
CSB-EP474129ENW
CSB-BP474129ENW
CSB-MP474129ENW
CSB-EP474129ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Serine--tRNA ligase (serS) CSB-YP474130ENW
CSB-EP474130ENW
CSB-BP474130ENW
CSB-MP474130ENW
CSB-EP474130ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0434 protein ycaR (ycaR) CSB-YP474131ENW
CSB-EP474131ENW
CSB-BP474131ENW
CSB-MP474131ENW
CSB-EP474131ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Heat shock protein hspQ (hspQ) CSB-YP474132ENW
CSB-EP474132ENW
CSB-BP474132ENW
CSB-MP474132ENW
CSB-EP474132ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli FMN reductase (NADH) RutF CSB-YP474133ENW
CSB-EP474133ENW
CSB-BP474133ENW
CSB-MP474133ENW
CSB-EP474133ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glyoxylate/hydroxypyruvate reductase A (ghrA) CSB-YP474134ENW
CSB-EP474134ENW
CSB-BP474134ENW
CSB-MP474134ENW
CSB-EP474134ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Flagellar P-ring protein (flgI) CSB-YP474135ENW
CSB-EP474135ENW
CSB-BP474135ENW
CSB-MP474135ENW
CSB-EP474135ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable septum site-determining protein MinC (minC) CSB-YP474136ENW
CSB-EP474136ENW
CSB-BP474136ENW
CSB-MP474136ENW
CSB-EP474136ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Peptidyl-tRNA hydrolase (pth) CSB-YP474137ENW
CSB-EP474137ENW
CSB-BP474137ENW
CSB-MP474137ENW
CSB-EP474137ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0259 membrane protein yciC (yciC), partial CSB-YP474138ENW1
CSB-EP474138ENW1
CSB-BP474138ENW1
CSB-MP474138ENW1
CSB-EP474138ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Gamma-aminobutyraldehyde dehydrogenase (prr) CSB-YP474139ENW
CSB-EP474139ENW
CSB-BP474139ENW
CSB-MP474139ENW
CSB-EP474139ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Spermidine export protein MdtI (mdtI), partial CSB-YP474140ENW1
CSB-EP474140ENW1
CSB-BP474140ENW1
CSB-MP474140ENW1
CSB-EP474140ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Electron transport complex protein RnfE (rnfE), partial CSB-YP474141ENW1
CSB-EP474141ENW1
CSB-BP474141ENW1
CSB-MP474141ENW1
CSB-EP474141ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cysteine desulfuration protein sufE (sufE) CSB-YP474142ENW
CSB-EP474142ENW
CSB-BP474142ENW
CSB-MP474142ENW
CSB-EP474142ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0249 protein ChbG (chbG) CSB-YP474143ENW
CSB-EP474143ENW
CSB-BP474143ENW
CSB-MP474143ENW
CSB-EP474143ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Arginine N-succinyltransferase (astA) CSB-YP474144ENW
CSB-EP474144ENW
CSB-BP474144ENW
CSB-MP474144ENW
CSB-EP474144ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0266 membrane protein yobD (yobD), partial CSB-YP474145ENW1
CSB-EP474145ENW1
CSB-BP474145ENW1
CSB-MP474145ENW1
CSB-EP474145ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>