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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodospirillum centenum NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP474166RLY
CSB-EP474166RLY
CSB-BP474166RLY
CSB-MP474166RLY
CSB-EP474166RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Phosphoglucosamine mutase (glmM) CSB-YP474167RLY
CSB-EP474167RLY
CSB-BP474167RLY
CSB-MP474167RLY
CSB-EP474167RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Protein-export protein secB (secB) CSB-YP474168RLY
CSB-EP474168RLY
CSB-BP474168RLY
CSB-MP474168RLY
CSB-EP474168RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Elongation factor 4 (lepA), partial CSB-YP474169RLY
CSB-EP474169RLY
CSB-BP474169RLY
CSB-MP474169RLY
CSB-EP474169RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Heme A synthase (ctaA), partial CSB-YP474170RLY
CSB-EP474170RLY
CSB-BP474170RLY
CSB-MP474170RLY
CSB-EP474170RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Chaperone protein DnaK (dnaK), partial CSB-YP474171RLY
CSB-EP474171RLY
CSB-BP474171RLY
CSB-MP474171RLY
CSB-EP474171RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP474172RLY
CSB-EP474172RLY
CSB-BP474172RLY
CSB-MP474172RLY
CSB-EP474172RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP474173RLY
CSB-EP474173RLY
CSB-BP474173RLY
CSB-MP474173RLY
CSB-EP474173RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP474174RLY
CSB-EP474174RLY
CSB-BP474174RLY
CSB-MP474174RLY
CSB-EP474174RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Cell division topological specificity factor (minE) CSB-YP474175RLY
CSB-EP474175RLY
CSB-BP474175RLY
CSB-MP474175RLY
CSB-EP474175RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Nitrogenase-stabilizing/protective protein nifW (nifW) CSB-YP474176RLY
CSB-EP474176RLY
CSB-BP474176RLY
CSB-MP474176RLY
CSB-EP474176RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP474177DXM
CSB-EP474177DXM
CSB-BP474177DXM
CSB-MP474177DXM
CSB-EP474177DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP474178DXM
CSB-EP474178DXM
CSB-BP474178DXM
CSB-MP474178DXM
CSB-EP474178DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP474179DXM1
CSB-EP474179DXM1
CSB-BP474179DXM1
CSB-MP474179DXM1
CSB-EP474179DXM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii DNA mismatch repair protein mutL (mutL), partial CSB-YP474180DXM
CSB-EP474180DXM
CSB-BP474180DXM
CSB-MP474180DXM
CSB-EP474180DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP474181DXM
CSB-EP474181DXM
CSB-BP474181DXM
CSB-MP474181DXM
CSB-EP474181DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP474182DXM
CSB-EP474182DXM
CSB-BP474182DXM
CSB-MP474182DXM
CSB-EP474182DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Leucine--tRNA ligase (leuS), partial CSB-YP474183DXM
CSB-EP474183DXM
CSB-BP474183DXM
CSB-MP474183DXM
CSB-EP474183DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii UPF0434 protein CbuG_1535 (CbuG_1535) CSB-YP474184DXM
CSB-EP474184DXM
CSB-BP474184DXM
CSB-MP474184DXM
CSB-EP474184DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Thiazole synthase (thiG) CSB-YP474185DXM
CSB-EP474185DXM
CSB-BP474185DXM
CSB-MP474185DXM
CSB-EP474185DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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