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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O157:H7 Enterobactin exporter EntS (entS), partial CSB-YP475526EOE
CSB-EP475526EOE
CSB-BP475526EOE
CSB-MP475526EOE
CSB-EP475526EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Integration host factor subunit alpha (ihfA) CSB-YP475527EOE
CSB-EP475527EOE
CSB-BP475527EOE
CSB-MP475527EOE
CSB-EP475527EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Cell division activator CedA (cedA) CSB-YP475528EOE
CSB-EP475528EOE
CSB-BP475528EOE
CSB-MP475528EOE
CSB-EP475528EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 N-succinylglutamate 5-semialdehyde dehydrogenase (astD) CSB-YP475529EOE
CSB-EP475529EOE
CSB-BP475529EOE
CSB-MP475529EOE
CSB-EP475529EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Chromosome initiation inhibitor (iciA) CSB-YP475530EOE
CSB-EP475530EOE
CSB-BP475530EOE
CSB-MP475530EOE
CSB-EP475530EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Putative Holliday junction resolvase (yqgF) CSB-YP475531EOE
CSB-EP475531EOE
CSB-BP475531EOE
CSB-MP475531EOE
CSB-EP475531EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP475532EOE
CSB-EP475532EOE
CSB-BP475532EOE
CSB-MP475532EOE
CSB-EP475532EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP475533EOE
CSB-EP475533EOE
CSB-BP475533EOE
CSB-MP475533EOE
CSB-EP475533EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0271 protein YbgL (ybgL) CSB-YP475534EOE
CSB-EP475534EOE
CSB-BP475534EOE
CSB-MP475534EOE
CSB-EP475534EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0266 membrane protein yobD (yobD), partial CSB-YP475535EOE1
CSB-EP475535EOE1
CSB-BP475535EOE1
CSB-MP475535EOE1
CSB-EP475535EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP475536EOE
CSB-EP475536EOE
CSB-BP475536EOE
CSB-MP475536EOE
CSB-EP475536EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Arginine--tRNA ligase (argS) CSB-YP475537EOE
CSB-EP475537EOE
CSB-BP475537EOE
CSB-MP475537EOE
CSB-EP475537EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP475538EOE1
CSB-EP475538EOE1
CSB-BP475538EOE1
CSB-MP475538EOE1
CSB-EP475538EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP475539EOE
CSB-EP475539EOE
CSB-BP475539EOE
CSB-MP475539EOE
CSB-EP475539EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Flagellar hook-basal body complex protein FliE (fliE) CSB-YP475540EOE
CSB-EP475540EOE
CSB-BP475540EOE
CSB-MP475540EOE
CSB-EP475540EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 D-tagatose-1,6-bisphosphate aldolase subunit kbaZ (kbaZ) CSB-YP475541EOE
CSB-EP475541EOE
CSB-BP475541EOE
CSB-MP475541EOE
CSB-EP475541EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Polyribonucleotide nucleotidyltransferase (pnp) CSB-YP475542EOE
CSB-EP475542EOE
CSB-BP475542EOE
CSB-MP475542EOE
CSB-EP475542EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP475543EOE
CSB-EP475543EOE
CSB-BP475543EOE
CSB-MP475543EOE
CSB-EP475543EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP475544EOE
CSB-EP475544EOE
CSB-BP475544EOE
CSB-MP475544EOE
CSB-EP475544EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein dsrB (dsrB) CSB-YP475545EOE
CSB-EP475545EOE
CSB-BP475545EOE
CSB-MP475545EOE
CSB-EP475545EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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