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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae SPS-sensor serine protease component SSY5 (SSY5) CSB-YP475326SVH
CSB-EP475326SVH
CSB-BP475326SVH
CSB-MP475326SVH
CSB-EP475326SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ER membrane protein complex subunit 3 (AIM27), partial CSB-YP475327SVH1
CSB-EP475327SVH1
CSB-BP475327SVH1
CSB-MP475327SVH1
CSB-EP475327SVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein AWRI1631_112270 (AWRI1631_112270), partial CSB-YP475328SVH
CSB-EP475328SVH
CSB-BP475328SVH
CSB-MP475328SVH
CSB-EP475328SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae SIT4-associating protein SAP190 (SAP190), partial CSB-YP475329SVH
CSB-EP475329SVH
CSB-BP475329SVH
CSB-MP475329SVH
CSB-EP475329SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 40S ribosomal protein S1-A (RPS1A) CSB-YP475330SVH
CSB-EP475330SVH
CSB-BP475330SVH
CSB-MP475330SVH
CSB-EP475330SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ATPase expression protein 1, mitochondrial (AEP1) CSB-YP475331SVH
CSB-EP475331SVH
CSB-BP475331SVH
CSB-MP475331SVH
CSB-EP475331SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial protein import protein ZIM17 (ZIM17) CSB-YP475332SVH
CSB-EP475332SVH
CSB-BP475332SVH
CSB-MP475332SVH
CSB-EP475332SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Adenylosuccinate synthetase (ADE12) CSB-YP475333SVH
CSB-EP475333SVH
CSB-BP475333SVH
CSB-MP475333SVH
CSB-EP475333SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial distribution and morphology protein 12 (MDM12) CSB-YP475334SVH
CSB-EP475334SVH
CSB-BP475334SVH
CSB-MP475334SVH
CSB-EP475334SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein SEY1 (SEY1), partial CSB-YP475335SVH1
CSB-EP475335SVH1
CSB-BP475335SVH1
CSB-MP475335SVH1
CSB-EP475335SVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Spindle pole component BBP1 (BBP1) CSB-YP475336SVH
CSB-EP475336SVH
CSB-BP475336SVH
CSB-MP475336SVH
CSB-EP475336SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Long-chain-alcohol oxidase FAO1 (FAO1), partial CSB-YP475337LQQ1
CSB-EP475337LQQ1
CSB-BP475337LQQ1
CSB-MP475337LQQ1
CSB-EP475337LQQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Calumenin-A (calua) CSB-YP475338SWI
CSB-EP475338SWI
CSB-BP475338SWI
CSB-MP475338SWI
CSB-EP475338SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Adenylosuccinate synthetase isozyme 1 A (adssl1a) CSB-YP475339SWI
CSB-EP475339SWI
CSB-BP475339SWI
CSB-MP475339SWI
CSB-EP475339SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Adipocyte plasma membrane-associated protein (apmap), partial CSB-YP475340SWI
CSB-EP475340SWI
CSB-BP475340SWI
CSB-MP475340SWI
CSB-EP475340SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Costars family protein C6orf115 homolog CSB-YP475341SWI
CSB-EP475341SWI
CSB-BP475341SWI
CSB-MP475341SWI
CSB-EP475341SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Enhancer of yellow 2 transcription factor homolog A (eny2-1) CSB-YP475342SWI
CSB-EP475342SWI
CSB-BP475342SWI
CSB-MP475342SWI
CSB-EP475342SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Partner of Y14 and mago A (wibga) CSB-YP475343SWI
CSB-EP475343SWI
CSB-BP475343SWI
CSB-MP475343SWI
CSB-EP475343SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii UPF0434 protein CBUD_1597.1 (CBUD_1597.1) CSB-YP475344DXO
CSB-EP475344DXO
CSB-BP475344DXO
CSB-MP475344DXO
CSB-EP475344DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Serine--tRNA ligase (serS) CSB-YP475345FNH
CSB-EP475345FNH
CSB-BP475345FNH
CSB-MP475345FNH
CSB-EP475345FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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