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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodospirillum centenum 30S ribosomal protein S4 (rpsD) CSB-YP475906RLY
CSB-EP475906RLY
CSB-BP475906RLY
CSB-MP475906RLY
CSB-EP475906RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Phosphopantetheine adenylyltransferase (coaD) CSB-YP475907RLY
CSB-EP475907RLY
CSB-BP475907RLY
CSB-MP475907RLY
CSB-EP475907RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Glutamate--tRNA ligase 2 (gltX2) CSB-YP475908RLY
CSB-EP475908RLY
CSB-BP475908RLY
CSB-MP475908RLY
CSB-EP475908RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Type III pantothenate kinase (coaX) CSB-YP475909RLY
CSB-EP475909RLY
CSB-BP475909RLY
CSB-MP475909RLY
CSB-EP475909RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP475910RLY
CSB-EP475910RLY
CSB-BP475910RLY
CSB-MP475910RLY
CSB-EP475910RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Trigger factor (tig) CSB-YP475911RLY
CSB-EP475911RLY
CSB-BP475911RLY
CSB-MP475911RLY
CSB-EP475911RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP475912RLY
CSB-EP475912RLY
CSB-BP475912RLY
CSB-MP475912RLY
CSB-EP475912RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum 60 kDa chaperonin (groL), partial CSB-YP475913RLY
CSB-EP475913RLY
CSB-BP475913RLY
CSB-MP475913RLY
CSB-EP475913RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Ribosome maturation factor RimP (rimP) CSB-YP475914RLY
CSB-EP475914RLY
CSB-BP475914RLY
CSB-MP475914RLY
CSB-EP475914RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP475915RLY
CSB-EP475915RLY
CSB-BP475915RLY
CSB-MP475915RLY
CSB-EP475915RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum 50S ribosomal protein L28 (rpmB) CSB-YP475916RLY
CSB-EP475916RLY
CSB-BP475916RLY
CSB-MP475916RLY
CSB-EP475916RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP475917RLY1
CSB-EP475917RLY1
CSB-BP475917RLY1
CSB-MP475917RLY1
CSB-EP475917RLY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP475918RLY
CSB-EP475918RLY
CSB-BP475918RLY
CSB-MP475918RLY
CSB-EP475918RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP475919RLY
CSB-EP475919RLY
CSB-BP475919RLY
CSB-MP475919RLY
CSB-EP475919RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP475920RLY
CSB-EP475920RLY
CSB-BP475920RLY
CSB-MP475920RLY
CSB-EP475920RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Alkyl hydroperoxide reductase AhpD (ahpD) CSB-YP475921DXM
CSB-EP475921DXM
CSB-BP475921DXM
CSB-MP475921DXM
CSB-EP475921DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii N utilization substance protein B homolog (nusB) CSB-YP475922DXM
CSB-EP475922DXM
CSB-BP475922DXM
CSB-MP475922DXM
CSB-EP475922DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP475923DXM
CSB-EP475923DXM
CSB-BP475923DXM
CSB-MP475923DXM
CSB-EP475923DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Chaperone protein DnaK (dnaK), partial CSB-YP475924DXM
CSB-EP475924DXM
CSB-BP475924DXM
CSB-MP475924DXM
CSB-EP475924DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Adenylosuccinate synthetase (purA) CSB-YP475925DXM
CSB-EP475925DXM
CSB-BP475925DXM
CSB-MP475925DXM
CSB-EP475925DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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