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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L1 (rplA) CSB-YP476506AWH
CSB-EP476506AWH
CSB-BP476506AWH
CSB-MP476506AWH
CSB-EP476506AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 30S ribosomal protein S10 (rpsJ) CSB-YP476507AWH
CSB-EP476507AWH
CSB-BP476507AWH
CSB-MP476507AWH
CSB-EP476507AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 30S ribosomal protein S19 (rpsS) CSB-YP476508AWH
CSB-EP476508AWH
CSB-BP476508AWH
CSB-MP476508AWH
CSB-EP476508AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L24 (rplX) CSB-YP476509AWH
CSB-EP476509AWH
CSB-BP476509AWH
CSB-MP476509AWH
CSB-EP476509AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L18 (rplR) CSB-YP476510AWH
CSB-EP476510AWH
CSB-BP476510AWH
CSB-MP476510AWH
CSB-EP476510AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L36 (rpmJ) CSB-YP476511AWH
CSB-EP476511AWH
CSB-BP476511AWH
CSB-MP476511AWH
CSB-EP476511AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Ribosome maturation factor RimP (rimP) CSB-YP476512AWH
CSB-EP476512AWH
CSB-BP476512AWH
CSB-MP476512AWH
CSB-EP476512AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP476513AWH
CSB-EP476513AWH
CSB-BP476513AWH
CSB-MP476513AWH
CSB-EP476513AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Tryptophan synthase beta chain (trpB) CSB-YP476514AWH
CSB-EP476514AWH
CSB-BP476514AWH
CSB-MP476514AWH
CSB-EP476514AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Peptidyl-tRNA hydrolase (pth) CSB-YP476515AWH
CSB-EP476515AWH
CSB-BP476515AWH
CSB-MP476515AWH
CSB-EP476515AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP476516AWH
CSB-EP476516AWH
CSB-BP476516AWH
CSB-MP476516AWH
CSB-EP476516AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Dihydroorotate dehydrogenase (quinone) CSB-YP476517AWH
CSB-EP476517AWH
CSB-BP476517AWH
CSB-MP476517AWH
CSB-EP476517AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Probable intracellular septation protein A (AFE_0667), partial CSB-YP476518AWH1
CSB-EP476518AWH1
CSB-BP476518AWH1
CSB-MP476518AWH1
CSB-EP476518AWH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans DNA ligase (ligA), partial CSB-YP476519AWH
CSB-EP476519AWH
CSB-BP476519AWH
CSB-MP476519AWH
CSB-EP476519AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Enolase (eno) CSB-YP476520AWH
CSB-EP476520AWH
CSB-BP476520AWH
CSB-MP476520AWH
CSB-EP476520AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans High frequency lysogenization protein HflD homolog (hflD) CSB-YP476521AWH
CSB-EP476521AWH
CSB-BP476521AWH
CSB-MP476521AWH
CSB-EP476521AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP476522AWH1
CSB-EP476522AWH1
CSB-BP476522AWH1
CSB-MP476522AWH1
CSB-EP476522AWH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP476523AWH
CSB-EP476523AWH
CSB-BP476523AWH
CSB-MP476523AWH
CSB-EP476523AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP476524AWH
CSB-EP476524AWH
CSB-BP476524AWH
CSB-MP476524AWH
CSB-EP476524AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP476525AWH
CSB-EP476525AWH
CSB-BP476525AWH
CSB-MP476525AWH
CSB-EP476525AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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