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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Thermodesulfovibrio yellowstonii Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP475506TMY
CSB-EP475506TMY
CSB-BP475506TMY
CSB-MP475506TMY
CSB-EP475506TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Non-canonical purine NTP pyrophosphatase (THEYE_A1837) CSB-YP475507TMY
CSB-EP475507TMY
CSB-BP475507TMY
CSB-MP475507TMY
CSB-EP475507TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP475508TMY
CSB-EP475508TMY
CSB-BP475508TMY
CSB-MP475508TMY
CSB-EP475508TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP475509TMY
CSB-EP475509TMY
CSB-BP475509TMY
CSB-MP475509TMY
CSB-EP475509TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii DNA ligase (ligA), partial CSB-YP475510TMY
CSB-EP475510TMY
CSB-BP475510TMY
CSB-MP475510TMY
CSB-EP475510TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii UPF0173 metal-dependent hydrolase THEYE_A0282 (THEYE_A0282) CSB-YP475511TMY
CSB-EP475511TMY
CSB-BP475511TMY
CSB-MP475511TMY
CSB-EP475511TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii DNA repair protein recO (recO) CSB-YP475512TMY
CSB-EP475512TMY
CSB-BP475512TMY
CSB-MP475512TMY
CSB-EP475512TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 50S ribosomal protein L27 (rpmA) CSB-YP475513TMY
CSB-EP475513TMY
CSB-BP475513TMY
CSB-MP475513TMY
CSB-EP475513TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii D-tyrosyl-tRNA (Tyr) deacylase CSB-YP475514TMY
CSB-EP475514TMY
CSB-BP475514TMY
CSB-MP475514TMY
CSB-EP475514TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 33 kDa chaperonin (hslO) CSB-YP475515TMY
CSB-EP475515TMY
CSB-BP475515TMY
CSB-MP475515TMY
CSB-EP475515TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 10 kDa chaperonin (groS) CSB-YP475516TMY
CSB-EP475516TMY
CSB-BP475516TMY
CSB-MP475516TMY
CSB-EP475516TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Ribosome maturation factor RimP (rimP) CSB-YP475517TMY
CSB-EP475517TMY
CSB-BP475517TMY
CSB-MP475517TMY
CSB-EP475517TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii tRNA (guanine-N (1)-)-methyltransferase CSB-YP475518TMY
CSB-EP475518TMY
CSB-BP475518TMY
CSB-MP475518TMY
CSB-EP475518TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Probable rRNA maturation factor (THEYE_A0868) CSB-YP475519TMY
CSB-EP475519TMY
CSB-BP475519TMY
CSB-MP475519TMY
CSB-EP475519TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Dihydrodipicolinate synthase (dapA) CSB-YP475520TMY
CSB-EP475520TMY
CSB-BP475520TMY
CSB-MP475520TMY
CSB-EP475520TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Probable S-methyl-5'-thioinosine phosphorylase CSB-YP475521TMY
CSB-EP475521TMY
CSB-BP475521TMY
CSB-MP475521TMY
CSB-EP475521TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP475522TMY
CSB-EP475522TMY
CSB-BP475522TMY
CSB-MP475522TMY
CSB-EP475522TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii NADH-quinone oxidoreductase subunit B 2 (nuoB2) CSB-YP475523TMY
CSB-EP475523TMY
CSB-BP475523TMY
CSB-MP475523TMY
CSB-EP475523TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 50S ribosomal protein L35 (rpmI) CSB-YP475524TMY
CSB-EP475524TMY
CSB-BP475524TMY
CSB-MP475524TMY
CSB-EP475524TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ureidoglycolate hydrolase (allA) CSB-YP475525EOE
CSB-EP475525EOE
CSB-BP475525EOE
CSB-MP475525EOE
CSB-EP475525EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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