Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Borrelia recurrentis Ribonuclease Z (rnz) CSB-YP475306BUK
CSB-EP475306BUK
CSB-BP475306BUK
CSB-MP475306BUK
CSB-EP475306BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Ribosome maturation factor RimP (rimP) CSB-YP475307BUK
CSB-EP475307BUK
CSB-BP475307BUK
CSB-MP475307BUK
CSB-EP475307BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Arginine deiminase (arcA) CSB-YP475308BUK
CSB-EP475308BUK
CSB-BP475308BUK
CSB-MP475308BUK
CSB-EP475308BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Probable rRNA maturation factor (BRE_63) CSB-YP475309BUK
CSB-EP475309BUK
CSB-BP475309BUK
CSB-MP475309BUK
CSB-EP475309BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis V-type proton ATPase subunit E (atpE) CSB-YP475310BUK
CSB-EP475310BUK
CSB-BP475310BUK
CSB-MP475310BUK
CSB-EP475310BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 30S ribosomal protein S2 (rpsB) CSB-YP475311BUK
CSB-EP475311BUK
CSB-BP475311BUK
CSB-MP475311BUK
CSB-EP475311BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 50S ribosomal protein L35 (rpmI) CSB-YP475312BUK
CSB-EP475312BUK
CSB-BP475312BUK
CSB-MP475312BUK
CSB-EP475312BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP475313BUK
CSB-EP475313BUK
CSB-BP475313BUK
CSB-MP475313BUK
CSB-EP475313BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 50S ribosomal protein L28 (rpmB) CSB-YP475314BUK
CSB-EP475314BUK
CSB-BP475314BUK
CSB-MP475314BUK
CSB-EP475314BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 30S ribosomal protein S12 (rpsL) CSB-YP475315BUK
CSB-EP475315BUK
CSB-BP475315BUK
CSB-MP475315BUK
CSB-EP475315BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 50S ribosomal protein L1 (rplA) CSB-YP475316BUK
CSB-EP475316BUK
CSB-BP475316BUK
CSB-MP475316BUK
CSB-EP475316BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Lipoprotein signal peptidase (lspA), partial CSB-YP475317BUK1
CSB-EP475317BUK1
CSB-BP475317BUK1
CSB-MP475317BUK1
CSB-EP475317BUK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Pheromone-processing carboxypeptidase KEX1 (KEX1), partial CSB-YP475318DIS
CSB-EP475318DIS
CSB-BP475318DIS
CSB-MP475318DIS
CSB-EP475318DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Vacuolar protein sorting/targeting protein PEP1 (PEP1), partial CSB-YP475319SVH
CSB-EP475319SVH
CSB-BP475319SVH
CSB-MP475319SVH
CSB-EP475319SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Exonuclease V, mitochondrial (EXO5), partial CSB-YP475320SVH
CSB-EP475320SVH
CSB-BP475320SVH
CSB-MP475320SVH
CSB-EP475320SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae tRNA (guanine-N (7)-)-methyltransferase subunit TRM82 CSB-YP475321SVH
CSB-EP475321SVH
CSB-BP475321SVH
CSB-MP475321SVH
CSB-EP475321SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Probable GDP-mannose transporter 2 (HVG1), partial CSB-YP475322SVH1
CSB-EP475322SVH1
CSB-BP475322SVH1
CSB-MP475322SVH1
CSB-EP475322SVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Increased recombination centers protein 6 (IRC6) CSB-YP475323SVH
CSB-EP475323SVH
CSB-BP475323SVH
CSB-MP475323SVH
CSB-EP475323SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Biogenesis of lysosome-related organelles complex 1 subunit KXD1 (KXD1) CSB-YP475324SVH
CSB-EP475324SVH
CSB-BP475324SVH
CSB-MP475324SVH
CSB-EP475324SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cell wall protein PIR5 (PIR5) CSB-YP475325SVH
CSB-EP475325SVH
CSB-BP475325SVH
CSB-MP475325SVH
CSB-EP475325SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>