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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L18 (rplR) CSB-YP474886AWI
CSB-EP474886AWI
CSB-BP474886AWI
CSB-MP474886AWI
CSB-EP474886AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 30S ribosomal protein S4 (rpsD) CSB-YP474887AWI
CSB-EP474887AWI
CSB-BP474887AWI
CSB-MP474887AWI
CSB-EP474887AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP474888AWI
CSB-EP474888AWI
CSB-BP474888AWI
CSB-MP474888AWI
CSB-EP474888AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP474889AWI
CSB-EP474889AWI
CSB-BP474889AWI
CSB-MP474889AWI
CSB-EP474889AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP474890AWI
CSB-EP474890AWI
CSB-BP474890AWI
CSB-MP474890AWI
CSB-EP474890AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Methionine--tRNA ligase (metG), partial CSB-YP474891AWI
CSB-EP474891AWI
CSB-BP474891AWI
CSB-MP474891AWI
CSB-EP474891AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 3-dehydroquinate synthase (aroB) CSB-YP474892AWI
CSB-EP474892AWI
CSB-BP474892AWI
CSB-MP474892AWI
CSB-EP474892AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Alanine racemase (alr) CSB-YP474893AWI
CSB-EP474893AWI
CSB-BP474893AWI
CSB-MP474893AWI
CSB-EP474893AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 30S ribosomal protein S16 (rpsP) CSB-YP474894AWI
CSB-EP474894AWI
CSB-BP474894AWI
CSB-MP474894AWI
CSB-EP474894AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Protein CrcB homolog (crcB), partial CSB-YP474895AWI1
CSB-EP474895AWI1
CSB-BP474895AWI1
CSB-MP474895AWI1
CSB-EP474895AWI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP474896AWI
CSB-EP474896AWI
CSB-BP474896AWI
CSB-MP474896AWI
CSB-EP474896AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP474897AWI
CSB-EP474897AWI
CSB-BP474897AWI
CSB-MP474897AWI
CSB-EP474897AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP474898AWI
CSB-EP474898AWI
CSB-BP474898AWI
CSB-MP474898AWI
CSB-EP474898AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP474899AWI
CSB-EP474899AWI
CSB-BP474899AWI
CSB-MP474899AWI
CSB-EP474899AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Indole-3-glycerol phosphate synthase (trpC) CSB-YP474900AWI
CSB-EP474900AWI
CSB-BP474900AWI
CSB-MP474900AWI
CSB-EP474900AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP474901AWI
CSB-EP474901AWI
CSB-BP474901AWI
CSB-MP474901AWI
CSB-EP474901AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Probable transcriptional regulatory protein VFMJ11_A0186 (VFMJ11_A0186) CSB-YP474902VFC
CSB-EP474902VFC
CSB-BP474902VFC
CSB-MP474902VFC
CSB-EP474902VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP474903VFC
CSB-EP474903VFC
CSB-BP474903VFC
CSB-MP474903VFC
CSB-EP474903VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri UPF0345 protein VFMJ11_A0567 (VFMJ11_A0567) CSB-YP474904VFC
CSB-EP474904VFC
CSB-BP474904VFC
CSB-MP474904VFC
CSB-EP474904VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP474905VFC
CSB-EP474905VFC
CSB-BP474905VFC
CSB-MP474905VFC
CSB-EP474905VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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