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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus Ribonuclease P protein component (rnpA) CSB-YP476446BQP
CSB-EP476446BQP
CSB-BP476446BQP
CSB-MP476446BQP
CSB-EP476446BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 33 kDa chaperonin (hslO) CSB-YP476447BQP
CSB-EP476447BQP
CSB-BP476447BQP
CSB-MP476447BQP
CSB-EP476447BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP476448BQP
CSB-EP476448BQP
CSB-BP476448BQP
CSB-MP476448BQP
CSB-EP476448BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L7/L12 (rplL) CSB-YP476449BQP
CSB-EP476449BQP
CSB-BP476449BQP
CSB-MP476449BQP
CSB-EP476449BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L22 (rplV) CSB-YP476450BQP
CSB-EP476450BQP
CSB-BP476450BQP
CSB-MP476450BQP
CSB-EP476450BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L5 (rplE) CSB-YP476451BQP
CSB-EP476451BQP
CSB-BP476451BQP
CSB-MP476451BQP
CSB-EP476451BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L30 (rpmD) CSB-YP476452BQP
CSB-EP476452BQP
CSB-BP476452BQP
CSB-MP476452BQP
CSB-EP476452BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L17 (rplQ) CSB-YP476453BQP
CSB-EP476453BQP
CSB-BP476453BQP
CSB-MP476453BQP
CSB-EP476453BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus NADPH dehydrogenase (namA) CSB-YP476454BQP
CSB-EP476454BQP
CSB-BP476454BQP
CSB-MP476454BQP
CSB-EP476454BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Protein RecA (recA) CSB-YP476455BQP
CSB-EP476455BQP
CSB-BP476455BQP
CSB-MP476455BQP
CSB-EP476455BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP476456BQP
CSB-EP476456BQP
CSB-BP476456BQP
CSB-MP476456BQP
CSB-EP476456BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Proline--tRNA ligase (proS), partial CSB-YP476457BQP
CSB-EP476457BQP
CSB-BP476457BQP
CSB-MP476457BQP
CSB-EP476457BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP476458BQP
CSB-EP476458BQP
CSB-BP476458BQP
CSB-MP476458BQP
CSB-EP476458BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus tRNA (guanine-N (1)-)-methyltransferase CSB-YP476459BQP
CSB-EP476459BQP
CSB-BP476459BQP
CSB-MP476459BQP
CSB-EP476459BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L28 (rpmB) CSB-YP476460BQP
CSB-EP476460BQP
CSB-BP476460BQP
CSB-MP476460BQP
CSB-EP476460BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP476461BQP
CSB-EP476461BQP
CSB-BP476461BQP
CSB-MP476461BQP
CSB-EP476461BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Dihydroorotase (pyrC) CSB-YP476462BQP
CSB-EP476462BQP
CSB-BP476462BQP
CSB-MP476462BQP
CSB-EP476462BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Redox-sensing transcriptional repressor rex (rex) CSB-YP476463BQP
CSB-EP476463BQP
CSB-BP476463BQP
CSB-MP476463BQP
CSB-EP476463BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP476464BQP
CSB-EP476464BQP
CSB-BP476464BQP
CSB-MP476464BQP
CSB-EP476464BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP476465BQP
CSB-EP476465BQP
CSB-BP476465BQP
CSB-MP476465BQP
CSB-EP476465BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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