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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cyanothece sp. ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP476646DZZ
CSB-EP476646DZZ
CSB-BP476646DZZ
CSB-MP476646DZZ
CSB-EP476646DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Protein-L-isoaspartate O-methyltransferase (pcm1) CSB-YP476647AZX
CSB-EP476647AZX
CSB-BP476647AZX
CSB-MP476647AZX
CSB-EP476647AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 30S ribosomal protein S18 (rpsR) CSB-YP476648AZX
CSB-EP476648AZX
CSB-BP476648AZX
CSB-MP476648AZX
CSB-EP476648AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 50S ribosomal protein L16 (rplP) CSB-YP476649AZX
CSB-EP476649AZX
CSB-BP476649AZX
CSB-MP476649AZX
CSB-EP476649AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP476650AZX
CSB-EP476650AZX
CSB-BP476650AZX
CSB-MP476650AZX
CSB-EP476650AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 50S ribosomal protein L32 (rpmF) CSB-YP476651AZX
CSB-EP476651AZX
CSB-BP476651AZX
CSB-MP476651AZX
CSB-EP476651AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Ribonuclease PH (rph1) CSB-YP476652AZX
CSB-EP476652AZX
CSB-BP476652AZX
CSB-MP476652AZX
CSB-EP476652AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Protein smg homolog (smg) CSB-YP476653AZX
CSB-EP476653AZX
CSB-BP476653AZX
CSB-MP476653AZX
CSB-EP476653AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Regulator of ribonuclease activity A (rraA) CSB-YP476654AZX
CSB-EP476654AZX
CSB-BP476654AZX
CSB-MP476654AZX
CSB-EP476654AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Phosphomethylpyrimidine synthase (thiC), partial CSB-YP476655AZX
CSB-EP476655AZX
CSB-BP476655AZX
CSB-MP476655AZX
CSB-EP476655AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP476656EYX
CSB-EP476656EYX
CSB-BP476656EYX
CSB-MP476656EYX
CSB-EP476656EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii 50S ribosomal protein L20 (rplT) CSB-YP476657EYX
CSB-EP476657EYX
CSB-BP476657EYX
CSB-MP476657EYX
CSB-EP476657EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP476658EYX
CSB-EP476658EYX
CSB-BP476658EYX
CSB-MP476658EYX
CSB-EP476658EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP476659EYX
CSB-EP476659EYX
CSB-BP476659EYX
CSB-MP476659EYX
CSB-EP476659EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP476660EYX
CSB-EP476660EYX
CSB-BP476660EYX
CSB-MP476660EYX
CSB-EP476660EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Enolase (eno) CSB-YP476661EYX
CSB-EP476661EYX
CSB-BP476661EYX
CSB-MP476661EYX
CSB-EP476661EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii 50S ribosomal protein L31 (rpmE) CSB-YP476662EYX
CSB-EP476662EYX
CSB-BP476662EYX
CSB-MP476662EYX
CSB-EP476662EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP476663EYX
CSB-EP476663EYX
CSB-BP476663EYX
CSB-MP476663EYX
CSB-EP476663EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP476664EYX
CSB-EP476664EYX
CSB-BP476664EYX
CSB-MP476664EYX
CSB-EP476664EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii ATP phosphoribosyltransferase (hisG) CSB-YP476665EYX
CSB-EP476665EYX
CSB-BP476665EYX
CSB-MP476665EYX
CSB-EP476665EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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