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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Dihydroxy-acid dehydratase (ilvD) CSB-YP475846ENW
CSB-EP475846ENW
CSB-BP475846ENW
CSB-MP475846ENW
CSB-EP475846ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0761 membrane protein yihY (yihY), partial CSB-YP475847ENW1
CSB-EP475847ENW1
CSB-BP475847ENW1
CSB-MP475847ENW1
CSB-EP475847ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ferrous-iron efflux pump FieF (fieF), partial CSB-YP475848ENW1
CSB-EP475848ENW1
CSB-BP475848ENW1
CSB-MP475848ENW1
CSB-EP475848ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP475849ENW
CSB-EP475849ENW
CSB-BP475849ENW
CSB-MP475849ENW
CSB-EP475849ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Pyridoxine kinase (pdxK) CSB-YP475850ENW
CSB-EP475850ENW
CSB-BP475850ENW
CSB-MP475850ENW
CSB-EP475850ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Dihydrodipicolinate synthase (dapA) CSB-YP475851ENW
CSB-EP475851ENW
CSB-BP475851ENW
CSB-MP475851ENW
CSB-EP475851ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cytoskeleton protein rodZ (rodZ), partial CSB-YP475852ENW
CSB-EP475852ENW
CSB-BP475852ENW
CSB-MP475852ENW
CSB-EP475852ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP475853ENW
CSB-EP475853ENW
CSB-BP475853ENW
CSB-MP475853ENW
CSB-EP475853ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L11 (rplK) CSB-YP475854ENW
CSB-EP475854ENW
CSB-BP475854ENW
CSB-MP475854ENW
CSB-EP475854ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Peptidase E (pepE) CSB-YP475855ENW
CSB-EP475855ENW
CSB-BP475855ENW
CSB-MP475855ENW
CSB-EP475855ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cation/acetate symporter ActP (actP), partial CSB-YP475856ENW
CSB-EP475856ENW
CSB-BP475856ENW
CSB-MP475856ENW
CSB-EP475856ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein RnfH (rnfH) CSB-YP475857ENW
CSB-EP475857ENW
CSB-BP475857ENW
CSB-MP475857ENW
CSB-EP475857ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Anaerobic nitric oxide reductase transcription regulator norR (norR) CSB-YP475858ENW
CSB-EP475858ENW
CSB-BP475858ENW
CSB-MP475858ENW
CSB-EP475858ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 5'/3'-nucleotidase SurE (surE) CSB-YP475859ENW
CSB-EP475859ENW
CSB-BP475859ENW
CSB-MP475859ENW
CSB-EP475859ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Adenylyl-sulfate kinase (cysC) CSB-YP475860ENW
CSB-EP475860ENW
CSB-BP475860ENW
CSB-MP475860ENW
CSB-EP475860ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP475861ENW
CSB-EP475861ENW
CSB-BP475861ENW
CSB-MP475861ENW
CSB-EP475861ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Trp operon repressor (trpR) CSB-YP475862ENW
CSB-EP475862ENW
CSB-BP475862ENW
CSB-MP475862ENW
CSB-EP475862ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli RNA pyrophosphohydrolase (rppH) CSB-YP475863ENW
CSB-EP475863ENW
CSB-BP475863ENW
CSB-MP475863ENW
CSB-EP475863ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli D-phenylhydantoinase (hyuA) CSB-YP475864ENW
CSB-EP475864ENW
CSB-BP475864ENW
CSB-MP475864ENW
CSB-EP475864ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribose-5-phosphate isomerase A (rpiA) CSB-YP475865ENW
CSB-EP475865ENW
CSB-BP475865ENW
CSB-MP475865ENW
CSB-EP475865ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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