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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Salmonella dublin Ribonuclease PH (rph) CSB-YP475066SWN
CSB-EP475066SWN
CSB-BP475066SWN
CSB-MP475066SWN
CSB-EP475066SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Carboxylate-amine ligase YbdK (ybdK) CSB-YP475067SWN
CSB-EP475067SWN
CSB-BP475067SWN
CSB-MP475067SWN
CSB-EP475067SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Uridine kinase (udk) CSB-YP475068SWN
CSB-EP475068SWN
CSB-BP475068SWN
CSB-MP475068SWN
CSB-EP475068SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Ribonuclease P protein component (rnpA) CSB-YP475069SWN
CSB-EP475069SWN
CSB-BP475069SWN
CSB-MP475069SWN
CSB-EP475069SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin HTH-type transcriptional regulator hdfR (hdfR) CSB-YP475070SWN
CSB-EP475070SWN
CSB-BP475070SWN
CSB-MP475070SWN
CSB-EP475070SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Glucosamine-6-phosphate deaminase (nagB) CSB-YP475071SWN
CSB-EP475071SWN
CSB-BP475071SWN
CSB-MP475071SWN
CSB-EP475071SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Potassium-transporting ATPase A chain (kdpA), partial CSB-YP475072SWN
CSB-EP475072SWN
CSB-BP475072SWN
CSB-MP475072SWN
CSB-EP475072SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Elongation factor P-like protein (yeiP) CSB-YP475073SWN
CSB-EP475073SWN
CSB-BP475073SWN
CSB-MP475073SWN
CSB-EP475073SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP475074SWN
CSB-EP475074SWN
CSB-BP475074SWN
CSB-MP475074SWN
CSB-EP475074SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Lipopolysaccharide core heptose (II)-phosphate phosphatase CSB-YP475075SWN
CSB-EP475075SWN
CSB-BP475075SWN
CSB-MP475075SWN
CSB-EP475075SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Protein FdhE (fdhE) CSB-YP475076SWN
CSB-EP475076SWN
CSB-BP475076SWN
CSB-MP475076SWN
CSB-EP475076SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0194 membrane protein ybhG (ybhG) CSB-YP475077SWN
CSB-EP475077SWN
CSB-BP475077SWN
CSB-MP475077SWN
CSB-EP475077SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF (arnF), partial CSB-YP475078SWN1
CSB-EP475078SWN1
CSB-BP475078SWN1
CSB-MP475078SWN1
CSB-EP475078SWN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Penicillin-insensitive murein endopeptidase (mepA) CSB-YP475079SWN
CSB-EP475079SWN
CSB-BP475079SWN
CSB-MP475079SWN
CSB-EP475079SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Cation-efflux pump FieF (fieF), partial CSB-YP475080SWN1
CSB-EP475080SWN1
CSB-BP475080SWN1
CSB-MP475080SWN1
CSB-EP475080SWN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP475081SWN
CSB-EP475081SWN
CSB-BP475081SWN
CSB-MP475081SWN
CSB-EP475081SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Integration host factor subunit beta (ihfB) CSB-YP475082SWN
CSB-EP475082SWN
CSB-BP475082SWN
CSB-MP475082SWN
CSB-EP475082SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Chromosome partition protein MukB (mukB), partial CSB-YP475083SWN
CSB-EP475083SWN
CSB-BP475083SWN
CSB-MP475083SWN
CSB-EP475083SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Ethanolamine ammonia-lyase light chain (eutC) CSB-YP475084SWN
CSB-EP475084SWN
CSB-BP475084SWN
CSB-MP475084SWN
CSB-EP475084SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP475085SWN
CSB-EP475085SWN
CSB-BP475085SWN
CSB-MP475085SWN
CSB-EP475085SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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