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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella dublin DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP475086SWN
CSB-EP475086SWN
CSB-BP475086SWN
CSB-MP475086SWN
CSB-EP475086SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Endonuclease V (nfi) CSB-YP475087SWN
CSB-EP475087SWN
CSB-BP475087SWN
CSB-MP475087SWN
CSB-EP475087SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP475088SWN1
CSB-EP475088SWN1
CSB-BP475088SWN1
CSB-MP475088SWN1
CSB-EP475088SWN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Methylglyoxal synthase (mgsA) CSB-YP475089SWN
CSB-EP475089SWN
CSB-BP475089SWN
CSB-MP475089SWN
CSB-EP475089SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP475090SWN
CSB-EP475090SWN
CSB-BP475090SWN
CSB-MP475090SWN
CSB-EP475090SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Peptidase B (pepB) CSB-YP475091SWN
CSB-EP475091SWN
CSB-BP475091SWN
CSB-MP475091SWN
CSB-EP475091SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP475092SWN
CSB-EP475092SWN
CSB-BP475092SWN
CSB-MP475092SWN
CSB-EP475092SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Flagellar hook-basal body complex protein FliE (fliE) CSB-YP475093SWN
CSB-EP475093SWN
CSB-BP475093SWN
CSB-MP475093SWN
CSB-EP475093SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Protein RnfH (rnfH) CSB-YP475094SWN
CSB-EP475094SWN
CSB-BP475094SWN
CSB-MP475094SWN
CSB-EP475094SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Primosomal replication protein n (priB) CSB-YP475095SWN
CSB-EP475095SWN
CSB-BP475095SWN
CSB-MP475095SWN
CSB-EP475095SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0307 protein YjgA (yjgA) CSB-YP475096SWN
CSB-EP475096SWN
CSB-BP475096SWN
CSB-MP475096SWN
CSB-EP475096SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP475097SWN
CSB-EP475097SWN
CSB-BP475097SWN
CSB-MP475097SWN
CSB-EP475097SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin S-ribosylhomocysteine lyase (luxS) CSB-YP475098SWN
CSB-EP475098SWN
CSB-BP475098SWN
CSB-MP475098SWN
CSB-EP475098SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Phosphoglycerol transferase I (mdoB), partial CSB-YP475099SWN1
CSB-EP475099SWN1
CSB-BP475099SWN1
CSB-MP475099SWN1
CSB-EP475099SWN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP475100SWN
CSB-EP475100SWN
CSB-BP475100SWN
CSB-MP475100SWN
CSB-EP475100SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0181 protein yoaH (yoaH) CSB-YP475101SWN
CSB-EP475101SWN
CSB-BP475101SWN
CSB-MP475101SWN
CSB-EP475101SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0260 protein ycgN (ycgN) CSB-YP475102SWN
CSB-EP475102SWN
CSB-BP475102SWN
CSB-MP475102SWN
CSB-EP475102SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP475103SWN
CSB-EP475103SWN
CSB-BP475103SWN
CSB-MP475103SWN
CSB-EP475103SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP475104SWN
CSB-EP475104SWN
CSB-BP475104SWN
CSB-MP475104SWN
CSB-EP475104SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Peptidyl-tRNA hydrolase (pth) CSB-YP475105SWN
CSB-EP475105SWN
CSB-BP475105SWN
CSB-MP475105SWN
CSB-EP475105SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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