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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella enteritidis PT4 UPF0761 membrane protein yihY (yihY), partial CSB-YP477486SWO1
CSB-EP477486SWO1
CSB-BP477486SWO1
CSB-MP477486SWO1
CSB-EP477486SWO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 HTH-type transcriptional activator rhaR (rhaR) CSB-YP477487SWO
CSB-EP477487SWO
CSB-BP477487SWO
CSB-MP477487SWO
CSB-EP477487SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 UvrABC system protein B (uvrB), partial CSB-YP477488SWO
CSB-EP477488SWO
CSB-BP477488SWO
CSB-MP477488SWO
CSB-EP477488SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Chromosome initiation inhibitor (iciA) CSB-YP477489SWO
CSB-EP477489SWO
CSB-BP477489SWO
CSB-MP477489SWO
CSB-EP477489SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Regulator of ribonuclease activity A (rraA) CSB-YP477490SWO
CSB-EP477490SWO
CSB-BP477490SWO
CSB-MP477490SWO
CSB-EP477490SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Soluble pyridine nucleotide transhydrogenase (sthA) CSB-YP477491SWO
CSB-EP477491SWO
CSB-BP477491SWO
CSB-MP477491SWO
CSB-EP477491SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP477492SWO
CSB-EP477492SWO
CSB-BP477492SWO
CSB-MP477492SWO
CSB-EP477492SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Dihydroorotase (pyrC) CSB-YP477493SWO
CSB-EP477493SWO
CSB-BP477493SWO
CSB-MP477493SWO
CSB-EP477493SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Glyoxylate/hydroxypyruvate reductase A (ghrA) CSB-YP477494SWO
CSB-EP477494SWO
CSB-BP477494SWO
CSB-MP477494SWO
CSB-EP477494SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Mannonate dehydratase (uxuA) CSB-YP477495SWO
CSB-EP477495SWO
CSB-BP477495SWO
CSB-MP477495SWO
CSB-EP477495SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP477496SWO
CSB-EP477496SWO
CSB-BP477496SWO
CSB-MP477496SWO
CSB-EP477496SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Endonuclease V (nfi) CSB-YP477497SWO
CSB-EP477497SWO
CSB-BP477497SWO
CSB-MP477497SWO
CSB-EP477497SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Maltoporin (lamB) CSB-YP477498SWO
CSB-EP477498SWO
CSB-BP477498SWO
CSB-MP477498SWO
CSB-EP477498SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Serine--tRNA ligase (serS) CSB-YP477499SWO
CSB-EP477499SWO
CSB-BP477499SWO
CSB-MP477499SWO
CSB-EP477499SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Cobyric acid synthase (cobQ), partial CSB-YP477500SWO
CSB-EP477500SWO
CSB-BP477500SWO
CSB-MP477500SWO
CSB-EP477500SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Bifunctional protein HldE (hldE) CSB-YP477501SWO
CSB-EP477501SWO
CSB-BP477501SWO
CSB-MP477501SWO
CSB-EP477501SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Putrescine aminotransferase (patA) CSB-YP477502SWO
CSB-EP477502SWO
CSB-BP477502SWO
CSB-MP477502SWO
CSB-EP477502SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Cation/acetate symporter ActP (actP), partial CSB-YP477503SWO
CSB-EP477503SWO
CSB-BP477503SWO
CSB-MP477503SWO
CSB-EP477503SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Chromosome partition protein mukF (mukF) CSB-YP477504SWO
CSB-EP477504SWO
CSB-BP477504SWO
CSB-MP477504SWO
CSB-EP477504SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 ATP phosphoribosyltransferase (hisG) CSB-YP477505SWO
CSB-EP477505SWO
CSB-BP477505SWO
CSB-MP477505SWO
CSB-EP477505SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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