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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella agona Peptide chain release factor 3 (prfC), partial CSB-YP477286SWK
CSB-EP477286SWK
CSB-BP477286SWK
CSB-MP477286SWK
CSB-EP477286SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Non-canonical purine NTP phosphatase (yjjX) CSB-YP477287SWK
CSB-EP477287SWK
CSB-BP477287SWK
CSB-MP477287SWK
CSB-EP477287SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP477288SWK
CSB-EP477288SWK
CSB-BP477288SWK
CSB-MP477288SWK
CSB-EP477288SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Biosynthetic arginine decarboxylase (speA), partial CSB-YP477289SWK
CSB-EP477289SWK
CSB-BP477289SWK
CSB-MP477289SWK
CSB-EP477289SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona UPF0235 protein yggU (yggU) CSB-YP477290SWK
CSB-EP477290SWK
CSB-BP477290SWK
CSB-MP477290SWK
CSB-EP477290SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP477291SWK
CSB-EP477291SWK
CSB-BP477291SWK
CSB-MP477291SWK
CSB-EP477291SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Undecaprenyl-diphosphatase (uppP), partial CSB-YP477292SWK1
CSB-EP477292SWK1
CSB-BP477292SWK1
CSB-MP477292SWK1
CSB-EP477292SWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP477293SWK
CSB-EP477293SWK
CSB-BP477293SWK
CSB-MP477293SWK
CSB-EP477293SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Electron transport complex protein RnfA (rnfA), partial CSB-YP477294SWK1
CSB-EP477294SWK1
CSB-BP477294SWK1
CSB-MP477294SWK1
CSB-EP477294SWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP477295SWK
CSB-EP477295SWK
CSB-BP477295SWK
CSB-MP477295SWK
CSB-EP477295SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona DnaA initiator-associating protein diaA (diaA) CSB-YP477296SWK
CSB-EP477296SWK
CSB-BP477296SWK
CSB-MP477296SWK
CSB-EP477296SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Chaperone protein DnaK (dnaK), partial CSB-YP477297SWK
CSB-EP477297SWK
CSB-BP477297SWK
CSB-MP477297SWK
CSB-EP477297SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Carnitine operon protein CaiE (caiE) CSB-YP477298SWK
CSB-EP477298SWK
CSB-BP477298SWK
CSB-MP477298SWK
CSB-EP477298SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Protein fixB (fixB) CSB-YP477299SWK
CSB-EP477299SWK
CSB-BP477299SWK
CSB-MP477299SWK
CSB-EP477299SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Putative phosphotransferase ydiA (ydiA) CSB-YP477300SWK
CSB-EP477300SWK
CSB-BP477300SWK
CSB-MP477300SWK
CSB-EP477300SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Succinylglutamate desuccinylase (astE) CSB-YP477301SWK
CSB-EP477301SWK
CSB-BP477301SWK
CSB-MP477301SWK
CSB-EP477301SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Sulfoxide reductase catalytic subunit YedY (yedY) CSB-YP477302SWK
CSB-EP477302SWK
CSB-BP477302SWK
CSB-MP477302SWK
CSB-EP477302SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 50S ribosomal protein L15 (rplO) CSB-YP477303SWK
CSB-EP477303SWK
CSB-BP477303SWK
CSB-MP477303SWK
CSB-EP477303SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 30S ribosomal protein S14 (rpsN) CSB-YP477304SWK
CSB-EP477304SWK
CSB-BP477304SWK
CSB-MP477304SWK
CSB-EP477304SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 50S ribosomal protein L16 (rplP) CSB-YP477305SWK
CSB-EP477305SWK
CSB-BP477305SWK
CSB-MP477305SWK
CSB-EP477305SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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