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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella schwarzengrund L-seryl-tRNA (Sec) selenium transferase CSB-YP476986SWV
CSB-EP476986SWV
CSB-BP476986SWV
CSB-MP476986SWV
CSB-EP476986SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 50S ribosomal protein L28 (rpmB) CSB-YP476987SWV
CSB-EP476987SWV
CSB-BP476987SWV
CSB-MP476987SWV
CSB-EP476987SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Tyrosine--tRNA ligase (tyrS) CSB-YP476988FMK
CSB-EP476988FMK
CSB-BP476988FMK
CSB-MP476988FMK
CSB-EP476988FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus 33 kDa chaperonin (hslO) CSB-YP476989FMK
CSB-EP476989FMK
CSB-BP476989FMK
CSB-MP476989FMK
CSB-EP476989FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Aspartate--tRNA ligase (aspS), partial CSB-YP476990FMK
CSB-EP476990FMK
CSB-BP476990FMK
CSB-MP476990FMK
CSB-EP476990FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus 50S ribosomal protein L9 (rplI) CSB-YP476991FMK
CSB-EP476991FMK
CSB-BP476991FMK
CSB-MP476991FMK
CSB-EP476991FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP476992FMK
CSB-EP476992FMK
CSB-BP476992FMK
CSB-MP476992FMK
CSB-EP476992FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus 30S ribosomal protein S14 (rpsN) CSB-YP476993FMK
CSB-EP476993FMK
CSB-BP476993FMK
CSB-MP476993FMK
CSB-EP476993FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP476994FMK
CSB-EP476994FMK
CSB-BP476994FMK
CSB-MP476994FMK
CSB-EP476994FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus GTP-sensing transcriptional pleiotropic repressor CodY (codY) CSB-YP476995FMK
CSB-EP476995FMK
CSB-BP476995FMK
CSB-MP476995FMK
CSB-EP476995FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP476996FMK
CSB-EP476996FMK
CSB-BP476996FMK
CSB-MP476996FMK
CSB-EP476996FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Galactose-6-phosphate isomerase subunit lacA (lacA) CSB-YP476997FMK
CSB-EP476997FMK
CSB-BP476997FMK
CSB-MP476997FMK
CSB-EP476997FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP476998FMK
CSB-EP476998FMK
CSB-BP476998FMK
CSB-MP476998FMK
CSB-EP476998FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Uronate isomerase (uxaC) CSB-YP476999FMK
CSB-EP476999FMK
CSB-BP476999FMK
CSB-MP476999FMK
CSB-EP476999FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Enolase (eno) CSB-YP477000FMK
CSB-EP477000FMK
CSB-BP477000FMK
CSB-MP477000FMK
CSB-EP477000FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus UPF0223 protein Sez_0908 (Sez_0908) CSB-YP477001FMK
CSB-EP477001FMK
CSB-BP477001FMK
CSB-MP477001FMK
CSB-EP477001FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Ribosome maturation factor RimM (rimM) CSB-YP477002FMK
CSB-EP477002FMK
CSB-BP477002FMK
CSB-MP477002FMK
CSB-EP477002FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus UvrABC system protein C (uvrC), partial CSB-YP477003FMK
CSB-EP477003FMK
CSB-BP477003FMK
CSB-MP477003FMK
CSB-EP477003FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Ribonuclease Z (rnz) CSB-YP477004FMK
CSB-EP477004FMK
CSB-BP477004FMK
CSB-MP477004FMK
CSB-EP477004FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus 50S ribosomal protein L11 (rplK) CSB-YP477005FMK
CSB-EP477005FMK
CSB-BP477005FMK
CSB-MP477005FMK
CSB-EP477005FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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